Mouse Heart Function
Mouse Heart Function - Related Videos
02:08
Viral Gene Delivery in the Mouse Heart for Evaluating Gene Effects on Heart Function
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2026
Source: Lu, A., et al. Viral Transgene Expression in Rodent Hearts and the Assessment of Cardiac Arrhythmia Risk. J. Vis. Exp. (2022)The video demonstrates a method for ultrasound-guided intramyocardial injection of a recombinant viral vector in a mouse model. This technique enables targeted gene delivery to the heart for studying the effects of transgene expression on cardiac function.
11:38
Assessment of Cardiac Function and Energetics in Isolated Mouse Hearts Using 31P NMR Spectroscopy
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Cited by 36 •
2010
Langendorff-mode isolated heart perfusion, in conjunction with 31P NMR spectroscopy, combines the fields of biochemistry and physiology into one experiment. The protocol allows for the dynamic measurement of high energy phosphate content and turnover in the heart while concurrently monitoring physiologic function. When performed correctly, this is a valuable technique in the assessment of cardiac energetics.
07:47
A Novel Ex vivo Culture Method for the Embryonic Mouse Heart
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Cited by 10 •
2013
Developmental studies in the mouse are hampered by the inaccessibility of the embryo during gestation. To promote the long-term culture of the embryonic heart at late stages of gestation, we developed a protocol in which the excised heart is cultured in a semi-solid, dilute Matrigel.
06:31
In Vitro Culture of Epicardial Cells From Mouse Embryonic Heart
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Cited by 11 •
2016
The epicardium is an essential source of multipotent cardiovascular progenitor cells and paracrine factors that are required for cardiovascular development and regeneration. We describe here a method to culture mouse embryonic epicardial cells.
07:49
A Pacing-Controlled Procedure for the Assessment of Heart Rate-Dependent Diastolic Functions in Murine Heart Failure Models
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2023
The present protocol describes obtaining the pressure-volume relationship through transesophageal pacing, which serves as a valuable tool in evaluating diastolic function in mouse models of heart failure.
FAQs
Pacemaker cells initiate electrical impulses, which move through the cardiac conduction system before reaching cardiomyocytes. This sequence coordinates atrial and ventricular activity rather than allowing chambers to contract independently. Calcium-dependent contraction then converts the electrical signal into force, helping generate the pressure needed for directed blood flow.
Calcium-dependent contraction is the link between electrical activation and mechanical performance. When an impulse reaches cardiomyocytes, the calcium-related contraction process enables them to develop force, so coordinated cellular activity becomes chamber pressure and circulation. In bioengineering studies, this link helps connect electrical measurements with changes in contractility and hemodynamics.
Researchers can examine how genetic or mechanical changes influence cardiac performance. The overview does not limit these changes to a single experimental design; instead, it identifies them as variables that can reveal altered rate, rhythm, contractility, or hemodynamics. This makes the mouse heart useful for connecting biological perturbations with measurable functional outcomes in bioengineering.
Researchers commonly combine electrocardiography, echocardiography, pressure measurements, and isolated-heart preparations. Together, these approaches provide complementary views of rate and rhythm, cardiac performance, pressure-related behavior, and hemodynamics. Using more than one measurement can help relate electrical activity to contraction and circulation, rather than relying on a single functional readout.
An isolated-heart preparation provides a distinct experimental setting for assessing cardiac performance. Researchers can pair it with pressure measurements and other functional readouts to examine contractility and hemodynamics. This approach is relevant when testing how a biomaterial, cardiac tissue construct, drug-delivery system, or engineered disease model affects heart performance.
It supplies functional endpoints for designing and testing biomaterials, cardiac tissue constructs, drug-delivery systems, and engineered disease models. Rather than evaluating these technologies only by their structure or composition, researchers can examine whether associated changes appear in rate, rhythm, contractility, or hemodynamics. The same framework also links mechanical or genetic changes to cardiovascular performance.