Nanoparticle Tracking Analysis
Nanoparticle Tracking Analysis - Related Videos
08:51
Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry
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Cited by 9 •
2013
A protocol for nanoparticle tracking analysis (NTA) and high-throughput flow cytometry to evaluate polymeric gene delivery nanoparticles is described. NTA is utilized to characterize the nanoparticle particle size distribution and the plasmid per particle distribution. High-throughput flow cytometry enables quantitative transfection efficacy evaluation for a library of gene delivery biomaterials.
09:19
Nanoparticle Tracking Analysis for the Quantification and Size Determination of Extracellular Vesicles
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Cited by 118 •
2021
We demonstrate how to use a novel nanoparticle tracking analysis instrument to estimate the size distribution and total particle concentration of extracellular vesicles isolated from mouse perigonadal adipose tissue and human plasma.
08:52
Improving Reproducibility to Meet Minimal Information for Studies of Extracellular Vesicles 2018 Guidelines in Nanoparticle Tracking Analysis
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Cited by 13 •
2021
Nanoparticle tracking analysis (NTA) is a widely used method to characterize extracellular vesicles. This paper highlights NTA experimental parameters and controls plus a uniform method of analysis and characterization of samples and diluents necessary to supplement the guidelines proposed by MISEV2018 and EV-TRACK for reproducibility between laboratories.
05:52
Analyzing the Movement of the Nauplius 'Artemia salina' by Optical Tracking of Plasmonic Nanoparticles
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Cited by 8 •
2014
We use optical tracking of plasmonic nanoparticles to probe and characterize the frequency movements of aquatic organisms.
07:08
Nanoparticle Tracking Analysis of Gold Nanoparticles in Aqueous Media through an Inter-Laboratory Comparison
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Cited by 11 •
2020
The protocol described here aims to measure the hydrodynamic diameter of spherical nanoparticles, more specifically gold nanoparticles, in aqueous media by means of Nanoparticle Tracking Analysis (NTA). The latter involves tracking the movement of particles due to Brownian motion and implementing the Stokes-Einstein equation to obtain the hydrodynamic diameter.
FAQs
Nanoparticle Tracking Analysis infers hydrodynamic diameter from how rapidly each illuminated particle undergoes Brownian motion. The recorded movement reflects diffusion, and the method applies the relationship between diffusion and particle size to calculate the diameter. Because the estimate comes from particle motion in liquid, the result describes hydrodynamic size rather than simply a geometric dimension.
Tracking scattered light from individual particles helps preserve information that a single bulk measurement could obscure. Particles within the same sample can contribute to different size populations, so individual-particle observations allow Nanoparticle Tracking Analysis to reveal heterogeneity. This is especially relevant when a biological or engineered material contains more than one particle population or changes during development.
Size distribution and concentration answer different questions about a liquid sample. Distribution shows how particle sizes are represented across the measured population, whereas concentration indicates how many particles are present. Considering both measurements helps distinguish a change in population abundance from a change in size composition, supporting interpretation of biological materials and engineered formulations.
A basic measurement starts with a liquid sample and laser illumination. The system records light scattered by individual particles, follows their Brownian motion, and uses the relationship between diffusion and particle size to calculate hydrodynamic diameters and particle concentration. The resulting measurements can then be examined for size distributions, heterogeneity, and changes in particle populations.
In bioengineering, researchers can apply Nanoparticle Tracking Analysis to extracellular vesicles, protein aggregates, liposomes, and other nanoparticle-based systems. The method supplies size and concentration information for comparing biological or engineered materials. Those measurements support characterization, quality control, and formulation development, while also contributing to studies of how such particle populations relate to biological transport.
By comparing samples, researchers can determine whether a material’s particle population remains consistent or shifts. Changes in size distribution may indicate altered population composition, while concentration changes reflect a different abundance of particles. Nanoparticle Tracking Analysis therefore helps identify heterogeneity and evaluate biological or engineered formulations using more than a single representative particle size.