Nanopore Drilling
Nanopore Drilling - Related Videos
08:51
Monitoring Protein Adsorption with Solid-state Nanopores
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Cited by 12 •
2011
A method of using solid-state nanopores to monitor the non-specific adsorption of proteins onto an inorganic surface is described. The method employs the resistive-pulse principle, allowing for the adsorption to be probed in real-time and at the single-molecule level. Because the process of single protein adsorption is far from equilibrium, we propose the employment of parallel arrays of synthetic nanopores, enabling for the quantitative determination of the apparent first-order reaction rate...
09:43
Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores
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Cited by 18 •
2013
A methodology for preparing solid-state nanopores in solution for biomolecular translocation experiments is presented. By applying short pulses of high electric fields, the nanopore diameter can be controllably enlarged with subnanometer precision and its electrical noise characteristics significantly improved. This procedure is performed in situ using standard laboratory equipment under experimental conditions.
07:33
Nanopore DNA Sequencing for Metagenomic Soil Analysis
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Cited by 8 •
2017
Nanopore technology for sequencing biomolecules has wide applications in the life sciences, including identification of pathogens, food safety monitoring, genomic analysis, metagenomic environmental monitoring, and characterization of bacterial antibiotic resistance. In this article, the procedure for metagenomic soil DNA sequencing for species identification using the nanopore sequencing technology is demonstrated.
05:28
Versatile Technique to Produce a Hierarchical Design in Nanoporous Gold
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2023
Nanoporous gold with a hierarchical and bimodal pore size distribution can be produced by combining electrochemical and chemical dealloying. The composition of the alloy can be monitored via EDS-SEM examination as the dealloying process advances. The material's loading capacity can be determined by studying protein adsorption onto the material.
04:26
Accessing the Porcine Brain via High-Speed Pneumatic Drill Craniectomy
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Cited by 2 •
2024
This protocol describes performing a craniectomy using a high-speed pneumatic drill on a 3-month-old Danish Landrace pig. The access is made through the frontal bone and reveals the ventral dura mater and underlying cerebral hemispheres. This procedure allows for access to a large portion of the pig brain.
FAQs
Pore diameter determines which ions or biomolecules can pass through the opening and helps establish the scale of molecular detection. Membrane thickness also affects electrical and molecular behavior, because transported species interact with the engineered structure over a defined distance. Controlling both dimensions allows researchers to tune nanopores for transport, separation, concentration, or single-molecule analysis.
Surface chemistry modifies how the nanopore interacts with ions and biomolecules. These chemical properties can influence transport through the opening and the electrical behavior measured during molecular passage. In bioengineering, adjusting the pore surface helps align the engineered membrane with a desired function, such as biomolecule detection, controlled transport, or development of an artificial membrane platform.
As DNA, RNA, or proteins pass through an engineered nanopore, their movement affects the pore’s electrical and molecular behavior. Monitoring these changes enables single-molecule analysis and supports biosensor development. The nanoscale opening is therefore not only a transport route but also a site where individual biomolecules can be detected as they move through the membrane.
Focused ion beams, electron beams, and other localized energy sources remove material from a thin membrane or solid material in a controlled way. This localized removal creates openings with defined diameters rather than relying on uncontrolled material loss. Such control is important because pore dimensions directly affect molecular transport, ion flow, and the resulting detection behavior.
A nanopore platform begins with selecting a thin membrane or solid material, followed by localized material removal using a focused ion beam, electron beam, or another energy source. Researchers then consider pore diameter, membrane thickness, and surface chemistry to obtain the intended electrical and molecular behavior. The resulting structure can be evaluated for transport, separation, concentration, or detection.
Researchers use Nanopore Drilling when they need engineered nanoscale openings for molecular transport or detection. Bioengineering applications include biosensors, single-molecule analysis, drug delivery systems, and artificial membrane platforms. The approach is particularly relevant when controlling ion flow or handling DNA, RNA, proteins, and other biomolecules at the scale of individual pores.
Engineered nanopores can regulate the movement of ions and biomolecules through precisely formed openings. By controlling pore dimensions and surface chemistry, researchers can influence how molecular species interact with and pass through the membrane. This capability supports concentration or separation of biomolecules and extends nanopore platforms beyond detection into broader bioengineering transport systems.
The technique supports functional membrane systems in which nanoscale transport and detection can be studied or applied. Outcomes may include biosensor architectures, single-molecule measurements, controlled drug delivery designs, and artificial membranes with engineered transport properties. In each case, the pore’s defined structure provides a controllable interface for studying ions and biomolecules.