Oxygen Barrier
Oxygen Barrier - Related Videos
08:56
Oxygen-Glucose Deprivation and Reoxygenation as an In Vitro Ischemia-Reperfusion Injury Model for Studying Blood-Brain Barrier Dysfunction
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Cited by 46 •
2015
Ischemia-Reperfusion (IR) injury is associated with a high rate of morbidity and mortality. The goal of the in vitro model of oxygen-glucose deprivation and reoxygenation (OGD-R) described here is to assess the effects of ischemia reperfusion injury on a variety of cells, particularly in blood-brain barrier (BBB) endothelial cells.
09:21
A Triple Culture Cell System Modeling the Human Blood-Brain Barrier
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Cited by 11 •
2021
This protocol describes a method to establish a human blood-brain barrier (BBB) in vitro model. The endothelial cells and pericytes are seeded on each side of an insert filter (blood compartment), and astrocytes are seeded in the bottom well (brain compartment). The model characterized was used for nanoparticles transport experiments.
09:10
Use of the MicroSiM (µSiM) Barrier Tissue Platform for Modeling the Blood-Brain Barrier
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Cited by 8 •
2024
This report provides protocols for assembly, cell culture, and assays on the µSiM platform for the construction of blood-brain barrier models.
08:58
Cerebral Blood Oxygenation Measurement Based on Oxygen-dependent Quenching of Phosphorescence
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Cited by 26 •
2011
We present an experimental procedure for measuring the partial pressure of oxygen (pO2) in cerebral vasculature based on oxygen-dependent quenching of phosphorescence. Animal preparation and imaging procedures were outlined for both large field of view CCD-based imaging of pO2 in rats and 2-photon excitation based imaging of pO2 in mice.
09:08
Detecting Reactive Oxygen Species
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2023
Reactive oxygen species are chemically active, oxygen-derived molecules capable of oxidizing other molecules. Because of their reactive nature, there are many deleterious effects associated with unchecked ROS production, including structural damage to DNA and other biological molecules. However, ROS can also be mediators of physiological signaling. There is accumulating evidence that ROS play significant roles in everything from activation of transcription factors to the mediation of...
FAQs
Performance reflects more than a material’s nominal ability to block gas. Oxygen first interacts with the material according to its solubility, then diffuses through it, while permeability captures the combined ease of transport. These properties determine how much oxygen crosses a barrier under a given concentration gradient, making them central for predicting exposure in biological or engineered systems.
Thickness, temperature, and concentration gradients can change oxygen transport even when the barrier material stays the same. Thickness alters the distance oxygen must traverse, temperature can affect transport behavior, and the concentration gradient provides the driving difference across the layer. Controlling these variables helps engineers tune exposure rather than treating barrier performance as a fixed material property.
In bioengineering, the goal is often controlled oxygen exposure rather than complete isolation. An oxygen barrier can moderate the oxygen environment near cells, tissues, implants, or biomaterials, helping maintain more stable conditions. The same transport control may also reduce oxygen exposure associated with oxidative damage or unwanted reactions, linking barrier design to biological compatibility and device function.
Design begins with the intended oxygen exposure and the component that must be protected or supplied. Engineers can then consider permeability, solubility, diffusion, layer thickness, temperature, and the concentration gradient together. This assessment helps determine whether the barrier should moderate delivery around a biological structure, limit exposure, or stabilize conditions within an engineered device.
Cell and tissue engineering can use these barriers to shape oxygen conditions around living or engineered structures. Applications include environments containing cells, tissues, implants, and biomaterials, where controlled oxygen movement supports stability or limits unwanted reactions. By tuning transport through the barrier, researchers can support more consistent engineered tissue conditions and improve the performance of bioengineered devices.
Oxygen barriers are useful when oxygen exposure must be limited to support preservation of an oxygen-sensitive product. Restricting oxygen movement can help control unwanted exposure, while the appropriate design still depends on permeability, diffusion, thickness, temperature, and the concentration gradient. This makes barrier selection relevant to preservation as well as biological and engineered-device applications.