Pars Plana Vitrectomy

Pars plana vitrectomy (PPV) is a microsurgical procedure that removes the vitreous gel from the eye to treat conditions affecting the retina and posterior segment. Through small ports placed in the pars plana, surgeons use a vitrector to cut and aspirate vitreous while balanced salt solution maintains intraocular pressure; illumination and forceps enable visualization and tissue manipulation. PPV supports treatment of retinal detachment, vitreous hemorrhage, epiretinal membranes, and complications of diabetic eye disease. In bioengineering, its development informs miniaturized surgical instruments, fluidics, intraocular visualization, and biomaterials designed to improve precision, safety, and postoperative healing.

Pars Plana Vitrectomy - Related Videos

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JoVE Journal - Biology
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PAR-CliP - A Method to Identify Transcriptome-wide the Binding Sites of RNA Binding Proteins

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Cited by 267 •

2010

RNA transcripts are subject to extensive posttranscriptional regulation that is mediated by a multitude of trans-acting RNA-binding proteins (RBPs). Here we present a generalizable method to identify precisely and on a transcriptome-wide scale the RNA binding sites of RBPs.

Subretinal Implantation of RPE on a Carrier in Minipigs: Guidelines for Preoperative Preparations, Surgical Techniques, and Postoperative Care

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Cited by 1 •

2022

The subretinal implantation of retinal pigmented epithelium (RPE) is one of the most promising approaches for the treatment of degenerative retinal diseases. However, the performance of preclinical studies on large-eye animal models remains challenging. This report presents guidelines for the subretinal transplantation of RPE on a cell carrier into minipigs.

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JoVE Journal - Medicine
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Biobanking of Human Aqueous and Vitreous Liquid Biopsies for Molecular Analyses

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Cited by 7 •

2023

This protocol presents an integrated biorepository platform for the standardized collection, annotation, and biobanking of high-quality human aqueous humor and vitreous liquid biopsies for molecular downstream analyses, including proteomics, metabolomics, and glycomics.

Research

JoVE Journal - Biology
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Efficient and Consistent Generation of Retinal Pigment Epithelium/Choroid Flatmounts from Human Eyes for Histological Analysis

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Cited by 1 •

2022

We describe a method to efficiently separate retinal pigment epithelium (RPE) from the retina in human eyes and generate whole RPE/choroid flatmounts for histological and morphometric analyses of the RPE.

Research

JoVE Journal - Medicine

Preparation Steps for Measurement of Reactivity in Mouse Retinal Arterioles Ex Vivo

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Cited by 12 •

2018

Many vision-threatening ocular diseases are associated with dysfunctional retinal microvessels. Therefore, the measurement of retinal arteriole responses is important to investigate the underlying pathophysiological mechanisms. This article describes a detailed protocol for mouse retinal arteriole isolation and preparation to assess the effects of vasoactive substances on vascular diameter.

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