Retinal Laser Surgery

Retinal laser surgery is a minimally invasive treatment that uses focused light energy to repair or stabilize damaged retinal tissue and preserve vision. In laser photocoagulation, a precisely controlled beam is directed through the eye to create small, localized thermal effects that seal leaking blood vessels, reinforce retinal tears, or reduce abnormal tissue growth while limiting damage to surrounding structures. The procedure supports care for conditions such as diabetic retinopathy, retinal tears, and selected vascular disorders, while also illustrating bioengineering principles in optical focusing, energy delivery, and tissue response. Advances in imaging and laser control continue to improve treatment precision and safety.

Retinal Laser Surgery - Related Videos

Research

JoVE EoE - Neuropathology

Modeling Retinal Degeneration and Regeneration in Zebrafish Using a Focal Laser Injury

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2025

This video demonstrates a procedure to induce retinal degeneration in adult zebrafish using laser injury. The laser targets photoreceptor cells in the retina, triggering cell death and a degenerative process. This damage activates Müller glia (MG) cells, leading to their dedifferentiation into stem cell progenitors that aid in retinal regeneration.

Müller Glia Cell Activation in a Laser-induced Retinal Degeneration and Regeneration Model in Zebrafish

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Cited by 14 •

2017

The zebrafish is a popular animal model to study mechanisms of retinal degeneration/regeneration in vertebrates. This protocol describes a method to induce localized injury disrupting the outer retina with minimal damage to the inner retina. Subsequently, we monitor in vivo the retinal morphology and the Müller glia response throughout retinal regeneration.

Research

JoVE Journal - Medicine
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A Step by Step Protocol for Subretinal Surgery in Rabbits

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Cited by 30 •

2016

Retinal pigment epithelium (RPE) replacement strategies and gene-based therapy are considered for several retinal degenerative conditions. For clinical translation, large eye animal models are required to study surgical techniques applicable in patients. Here we present a rabbit model for subretinal surgery geared towards RPE transplantation, which is versatile and cost-efficient.

Research

JoVE Journal - Neuroscience

Preparation of Retinal Samples for Volume Electron Microscopy

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2024

This protocol describes the detailed steps for preparing retinal samples for volume electron microscopy, focusing on the structural features of retinal photoreceptor terminals.

Preparing Retinal Organoid Samples for Transmission Electron Microscopy

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Cited by 1 •

2024

This protocol provides an optimized and elaborate preparation procedure for retinal organoid samples for transmission electron microscopy. It is suitable for applications that involve the analysis of synapses in mature retinal organoids.

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