Small Intestinal Submucosa
Small Intestinal Submucosa - Related Videos
04:52
Isolating Enteric Glial Cells From the Submucosa and Lamina Propria of a Mouse
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2025
This video demonstrates a method for isolating enteric glial cells from the submucosa and lamina propria of the mouse intestine. It outlines the steps involved in extracting submucosa and lamina propria tissue, isolating the enteric glial cells from them, and culturing the cells on a coated well-plate to establish an enteric glial cell culture.
10:58
A Protocol for Lentiviral Transduction and Downstream Analysis of Intestinal Organoids
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Cited by 81 •
2015
In this video protocol we give a step by step explanation of lentiviral transduction in organoids of primary intestinal epithelium and of processing and downstream analysis of these cultures by quantitative RT-PCR, RNA-microarray and immunohistochemistry.
09:44
Functional Assessment of Intestinal Motility and Gut Wall Inflammation in Rodents: Analyses in a Standardized Model of Intestinal Manipulation
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Cited by 32 •
2012
Postoperative ileus (POI) is a complication of abdominal surgery leading to increased morbidity and a prolonged hospital stay. Because prophylactic or therapeutic strategies are lacking intensified research is necessary. Therefore we established a standardized and feasible mouse model to investigate the pathophysiology of POI and to study potential therapeutic options.
06:38
A Novel Method for the Culture and Polarized Stimulation of Human Intestinal Mucosa Explants
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Cited by 16 •
2013
We introduce a novel method for the maintenance of human intestinal mucosa in culture and monitoring of the response to various types of stimuli over at least 24 hrs. With our method, the polarity of the tissue is maintained, allowing for a physiological stimulation via the apical route.
05:38
Generation of Apical-Out Intestinal Organoids and Assessment of Organoid Proliferation Rate Using EdU-Labeling
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Cited by 1 •
2025
Here, we describe a protocol for generating apical-out intestinal organoids from standard matrix-embedded organoid cultures. It also outlines the subsequent incorporation of EdU into actively proliferating cells and the semiautomatic quantification of EdU-positive cells.
FAQs
Decellularization does more than remove cells from SIS; it aims to retain the native matrix architecture, structural proteins, and signaling molecules. These preserved features create a substrate for cell attachment and migration while leaving the implanted material available for host-driven remodeling. In bioengineering, maintaining this matrix organization is therefore central to biological performance after implantation.
The collagen-rich matrix provides structural support, while retained signaling molecules can influence how surrounding cells attach, migrate, and participate in tissue formation. This combination distinguishes SIS from a material that supplies only physical support. For engineered constructs, the balance between preserved biological cues and the scaffold’s form helps determine whether it can guide new tissue formation at the intended site.
SIS performance depends on more than its composition. Processing can affect the preserved matrix, while the implantation site determines the biological environment in which remodeling occurs. Mechanical demands also matter, because a construct suitable for one tissue may not meet the requirements of another. These variables explain why the same scaffold format can produce different outcomes across applications.
It combines biodegradability with a matrix that can support cell attachment, migration, and host-driven remodeling. Synthetic materials may offer an alternative scaffold strategy, but SIS is studied specifically for its preserved biological architecture and signaling content. Its usefulness therefore depends on whether the target repair benefits from biological guidance as well as structural support.
A high-level preparation workflow begins with intestinal tissue, often from porcine sources, followed by decellularization to remove most cellular components while preserving extracellular matrix features. The resulting material can then be configured as a sheet, tube, or three-dimensional scaffold. This sequence links biological processing with the physical form needed for a particular regenerative application.
SIS can be configured as sheets, tubes, or three-dimensional scaffolds, allowing the material to match different repair geometries. Sheets are relevant to skin repair, tubular forms to blood vessels or urinary tissues, and three-dimensional constructs to tissue-engineering designs, including gastrointestinal structures. Format selection connects the scaffold’s physical organization with the anatomy and demands of the repair.
In bioengineering, SIS provides a biodegradable framework that can support regeneration rather than serving only as a permanent replacement. As the material remodels, host cells can use its matrix environment for attachment and migration, while new tissue forms. This makes it useful for studying regenerative repairs in skin, vascular, urinary, and gastrointestinal applications, with outcomes shaped by site and mechanics.