Thrombus Growth Kinetics
Thrombus Growth Kinetics - Related Videos
09:01
Intravital Microscopy and Thrombus Induction in the Earlobe of a Hairless Mouse
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Cited by 4 •
2017
The ear model of the hairless SKH1-Hrhr mouse enables intravital fluorescence microscopy of microcirculation and phototoxic thrombus induction without prior surgical preparation in the examined microvascular bed. Therefore, the ear of the hairless mouse is an excellent in vivo model to study the complex interactions during microvascular thrombus formation, thrombus evolution, and thrombolysis.
05:54
Intravital Fluorescence Microscopy to Study Microvascular Thrombus Formation
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2025
This video demonstrates the intravital fluorescence microscopy technique to study thrombus formation in a mouse model. The mouse is injected with a fluorophore-labeled polysaccharide to monitor blood circulation. Upon illumination with a high-intensity light, the phototoxic induction of an injury in the microvasculature of the ear leads to the formation of a thrombus, which is confirmed by visualizing the obstruction in the blood flow.
02:05
Visualization of a Cerebral Thrombus in a Mouse Using Micro-Computed Tomography Imaging
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2025
Source: Kim, D., et. al. Combined Near-infrared Fluorescent Imaging and Micro-computed Tomography for Directly Visualizing Cerebral Thromboemboli. J. Vis. Exp. (2016)This video demonstrates visualization of cerebral thrombus in mice using micro-computed tomography (micro-CT) imaging. A cerebral thrombus mouse model is injected with fibrin-targeted gold nanoparticles acting as a contrasting agent. The mouse is placed on the bed of a micro-CT machine, and images are acquired from multiple angles.
00:23
Kinetic Energy
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2019
Kinetic energy is the ability of an object in motion to do work or enact change. It can take on many forms. For instance, water flowing down a waterfall has kinetic energy. In biological systems, particles of light travel and are absorbed by plants to create chemical energy. Animals consume the chemical energy and give off molecules that carry their scent through the air. They also generate kinetic energy when they run away from predators. Entire systems also possess kinetic energy, like the...
01:19
Enzyme Kinetics
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2019
Enzymes speed up reactions by lowering the activation energy of the reactants. The speed at which the enzyme turns reactants into products is called the rate of reaction. Several factors impact the rate of reaction, including the number of available reactants. Enzyme kinetics is the study of how an enzyme changes the rate of a reaction. Scientists typically study enzyme kinetics with a fixed amount of enzyme in the controlled environment of a test tube. When more reactant, or substrate, is...
FAQs
Platelet adhesion to an injured vessel or blood-contacting material provides the initial surface for clot development. Once attached, platelets activate and recruit additional platelets, increasing the growing mass. The balance between adhesion, activation, and recruitment affects how quickly the thrombus expands and helps determine its developing structure under the tested vascular or engineered conditions.
Thrombin converts fibrinogen into fibrin, producing a network that stabilizes the platelet-rich structure. This biochemical step changes the clot from a collection of recruited platelets into a more mechanically supported thrombus. Measuring growth alongside fibrin formation can therefore help distinguish early platelet accumulation from later stabilization during studies of clot behavior.
Flow influences how platelets and soluble coagulation components reach the developing thrombus. Transport conditions can consequently change both the rate of growth and its spatial pattern, rather than affecting only the final clot size. Bioengineering models must therefore account for blood-flow conditions when comparing thrombus formation across experiments, devices, or material surfaces.
Tracking growth over time allows researchers to compare how rapidly thrombi develop and where accumulation occurs. Differences in these rates or spatial patterns may reflect altered platelet recruitment, fibrin stabilization, or transport conditions. Such comparisons provide a quantitative way to examine how vascular injury, blood-contacting materials, or engineered flow environments influence clot behavior.
A study typically establishes blood flow through a microfluidic environment, exposes the blood to the relevant vascular or material condition, and follows thrombus formation over time. Quantitative analysis then evaluates growth rates and spatial patterns under the selected transport conditions. This workflow enables controlled comparisons of clot behavior while supporting bioengineering investigations of flow and surface effects.
Measurements of thrombus formation are useful when a vascular implant or biomaterial will contact blood and could influence platelet adhesion, activation, or fibrin stabilization. Comparing growth behavior under controlled conditions helps assess thrombosis risk during material or device evaluation. The resulting evidence can guide design decisions before broader studies of blood compatibility or performance.
Quantitative growth measurements provide an outcome for examining whether an anticoagulant treatment changes clot development under defined blood-flow conditions. Researchers can compare the rate or spatial pattern of thrombus formation between experimental conditions, linking treatment exposure to observable changes in clot behavior. This approach complements bioengineering models used to study therapy performance and thrombosis risk.