Tumor Stromal
Tumor Stromal - Related Videos
07:20
A Novel Stromal Fibroblast-Modulated 3D Tumor Spheroid Model for Studying Tumor-Stroma Interaction and Drug Discovery
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Cited by 21 •
2020
A novel three-dimensional spheroid model based on the heterotypic interaction of tumor cells and stromal fibroblasts is established. Here, we present coculture of tumor cells and stromal fibroblasts, time-lapse imaging, and confocal microscopy to visualize the formation of spheroids. This three-dimensional model offers a pertinent platform to study tumor-stroma interactions and test cancer therapeutics.
01:24
Protein Transport to the Stroma
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2023
Chloroplasts are triple membrane structures with an outer membrane, an inner membrane, and a thylakoid membrane, each containing distinct metabolite transporters, membrane translocons, and enzymes. Appropriate sorting and translocating these proteins to their correct membrane systems is essential for chloroplast function. Protein complexes called the translocon of the outer chloroplast membrane or TOC complex, and the translocon of the inner chloroplast membrane or TIC complex mediate the...
08:40
A Biomimetic Model for Liver Cancer to Study Tumor-Stroma Interactions in a 3D Environment with Tunable Bio-Physical Properties
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Cited by 22 •
2020
This protocol presents a 3D biomimetic model with accompanying fibrotic stromal compartment. Prepared with physiologically relevant hydrogels in ratios mimicking the bio-physical properties of the stromal extracellular matrix, an active mediator of cellular interactions, tumor growth and metastasis.
08:04
Evaluation of Nanoparticle Uptake in Tumors in Real Time Using Intravital Imaging
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Cited by 51 •
2011
We present a novel approach to quantify nanoparticle localization in the vasculature of human xenografted tumors using dynamic, real-time intravital imaging in an avian embryo model.
08:42
Establishment of a Co-culture System of Patient-Derived Colorectal Tumor Organoids and Tumor-Infiltrating Lymphocytes (TILs)
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Cited by 4 •
2025
The protocol describes a method for creating a patient-derived colorectal cancer (CRC) organoid model co-cultured with tumor-infiltrating lymphocytes (TILs) to study their interactions and therapeutic potential. This model provides a preclinical platform to explore immune responses in the tumor microenvironment and to predict the efficacy of TIL-based therapy for personalized CRC treatment.
FAQs
Stromal fibroblasts influence malignant behavior through extracellular-matrix deposition and growth-factor signaling. By changing the surrounding matrix and local signaling environment, they can affect how tumor cells grow, invade nearby tissue, and respond to treatment. Bioengineered models that include fibroblasts therefore provide a way to examine cancer progression as an interaction between malignant cells and their supporting surroundings.
Endothelial cells contribute to angiogenesis, the formation of blood vessels within or around a tumor. This vascular activity is a key stromal process because it changes the tumor microenvironment and can influence tumor growth and treatment response. Including endothelial components in engineered systems helps researchers study how vessel-associated interactions contribute to disease behavior rather than examining cancer cells alone.
Immune cells participate in tumor stromal signaling and immune modulation, meaning they can change the local immune environment surrounding malignant cells. Their interactions with cancer cells and other stromal components may influence tumor progression and treatment response. Engineered tumor models can incorporate these interactions to investigate how the microenvironment affects cancer biology and to identify stromal pathways that may be therapeutically relevant.
These platforms recreate tumor stromal interactions in complementary ways. Three-dimensional cultures model cellular organization beyond a flat culture surface, organ-on-a-chip systems recreate interactions within an engineered platform, and engineered extracellular matrices focus on the surrounding structural environment. Together, they allow researchers to study tumor progression, invasion, signaling, and drug resistance under more biologically relevant conditions.
A typical approach is to recreate interactions among malignant cells, stromal fibroblasts, endothelial cells, immune cells, extracellular matrix, and signaling factors within a three-dimensional or engineered system. Researchers then examine outcomes such as tumor growth, invasion, and treatment response. This workflow connects defined microenvironmental components with measurable cancer behaviors and supports comparison of different stromal conditions.
These models are useful when treatment response depends on interactions between cancer cells and their surrounding microenvironment. Because stromal components can contribute to drug resistance, engineered systems may provide more predictive therapeutic testing than models focused only on malignant cells. They also help researchers identify stromal pathways that could be targeted alongside cancer cells to improve treatment strategies.