Vascular Endothelial Cells
Vascular Endothelial Cells - Related Videos
06:15
Evaluation of Bioenergetic Function in Cerebral Vascular Endothelial Cells
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Cited by 10 •
2016
Endothelial cell mitochondria are critical to maintain blood-brain-barrier integrity. We introduce a protocol to measure bioenergetic function in cerebral vascular endothelial cells.
02:43
Assessing Mitochondrial Function in Cerebral Vascular Endothelial Cells
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2025
This video demonstrates the assessment of the mitochondrial functioning of cerebral vascular endothelial (CVE) cells using an extracellular flux assay. CVE cells are plated in an extracellular flux cell culture plate. Extracellular flux sensors are hydrated before loading and calibration into the bioanalyzer. The plate containing cells is then loaded for analysis.
09:00
Isolation and Identification of Vascular Endothelial Cells from Distinct Adipose Depots for Downstream Applications
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Cited by 3 •
2022
This protocol details a method for the dissection of mouse adipose depots and the isolation and digestion of respective arteries to liberate and then identify the endothelial cell population. Freshly isolated cells used in downstream applications will advance the understanding of vascular cell biology and the mechanisms of vascular dysfunction.
12:41
A Novel Mammary Fat Pad Transplantation Technique to Visualize the Vessel Generation of Vascular Endothelial Stem Cells
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Cited by 1 •
2017
This work demonstrates a novel approach to assess the proliferation, differentiation, and vessel-forming potential of vascular endothelial stem cells (VESCs) through mammary fat pad transplantation followed by whole-mount tissue preparation for microscopic observation. A lineage tracing strategy to investigate the behavior of VESCs in vivo is also presented.
07:26
Stimulation of Vascular Endothelial Cells Using Neutrophil Extracellular Traps in the Presence of Low-Density Lipoprotein
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2025
A series of methods is described, including the isolation of low-density lipoprotein (LDL) from human plasma, differentiation of HL-60 cells into neutrophil-like cells, preparation of neutrophil extracellular traps (NETs) in the presence of LDL, and stimulation of human aortic endothelial cells with a mixture of NETs and LDL.
FAQs
Their cell-cell junctions regulate how easily substances pass between the bloodstream and surrounding tissue. Biochemical signals and mechanical inputs can alter junctional behavior, allowing permeability to increase or decrease as vascular conditions change. This adjustable barrier is important for bioengineered vessel models that aim to reproduce controlled exchange between blood and tissues.
Fluid shear stress provides a mechanical signal generated by blood flow. Vascular endothelial cells respond to this force as part of their regulation of blood flow, barrier function, and vessel formation. Including flow-related mechanical conditions in bioengineered systems therefore helps researchers evaluate cellular responses in environments that more closely represent vascular physiology.
Signaling pathways coordinate how vascular endothelial cells respond when new vessel growth is needed. Their activity connects biochemical cues with changes in cell behavior that promote angiogenesis, the formation of new blood vessels. Studying these responses helps bioengineers examine how engineered environments may support or regulate vascular development in tissues.
In bioengineering, vascular endothelial cells can be incorporated into blood vessel models to examine barrier behavior, responses to biochemical and mechanical cues, and vessel-forming activity. These models provide a controlled setting for studying vascular function and for comparing how engineered conditions influence cellular outcomes without relying only on observations from intact biological vessels.
Researchers use these cells to assess how biomaterials interact with vascular lining cells and to examine implant compatibility. Changes in permeability, flow-related responses, inflammation-related behavior, or vessel formation can provide relevant biological readouts. Such testing helps identify whether an engineered material or implant supports vascular conditions appropriate for its intended use.
Their ability to regulate vessel formation makes vascular endothelial cells valuable for strategies intended to vascularize engineered tissues. Incorporating their responses into tissue-engineering designs can help researchers study whether a construct develops vessel-related functions and interfaces appropriately with surrounding tissue. This work is relevant to efforts aimed at building more functional tissue-engineered organs.