Opti-mem
Opti-mem - Related Videos
08:31
Three-dimensional Optical-resolution Photoacoustic Microscopy
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Cited by 27 •
2011
Optical-resolution photoacoustic microscopy (OR-PAM) is an emerging technology capable of imaging optical absorption contrasts in vivo with cellular resolution and sensitivity. Here, we provide a visualized instruction on the experimental protocols of OR-PAM, including system configuration, system alignment, typical in vivo experimental procedures, and functional imaging schemes.
12:11
Using Optical Tweezers for the Generation of Hybrid Spheroids
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2025
In this paper, we propose a protocol for hybrid spheroid manufacturing using optical tweezers. This method allows precise control of the early stages of the spheroid formation process, which is unattainable by other methods.
10:13
Drosophila Preparation and Longitudinal Imaging of Heart Function In Vivo Using Optical Coherence Microscopy (OCM)
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Cited by 23 •
2016
Here, the experimental protocols are described for preparing Drosophila at different developmental stages and performing longitudinal optical imaging of Drosophila heartbeats using a custom optical coherence microscopy (OCM) system. The cardiac morphological and dynamical changes can be quantitatively characterized by analyzing the heart structural and functional parameters from OCM images.
09:39
Optical Biosensing
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2023
Optical biosensors utilize light to detect the binding of a target molecule. These sensors can utilize a label molecule, which produces a measurable signal such as fluorescence, or these sensors can be label-free and use the changes in optical properties, such as refractive index, to sense for the binding of the target molecule. This video introduces both label and label-free optical biosensors, demonstrates their use in the laboratory, and shows some applications of the technology.
08:53
Mesoscopic Optical Imaging of Whole Mouse Heart
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Cited by 6 •
2021
We report a method for mesoscopic reconstruction of the whole mouse heart by combining new advancements in tissue transformation and staining with the development of an axially scanned light-sheet microscope.
FAQs
Opti-MEM’s low-protein environment can favor the formation and delivery of nucleic acid complexes during transfection. With less serum present, researchers can prepare these complexes in a more controlled setting. This is particularly relevant when plasmid DNA or RNA must reach mammalian cells for gene-expression experiments.
The balanced nutrients and salts in Opti-MEM help maintain mammalian cells while experiments are performed under reduced-serum conditions. That support matters because cells must remain suitable for manipulation, not merely survive handling. Maintaining this consistent environment helps researchers compare gene-expression experiments while limiting differences associated with serum content.
Reduced-serum conditions can reduce serum-related variability in cell-based assays, making the experimental environment more consistent across measurements. This is useful when differences in gene expression or cellular responses need to be attributed to the tested manipulation rather than changing serum contributions. Opti-MEM therefore supports cleaner comparisons during optimization and routine experimental work.
It can serve both as a medium for mammalian cell handling and as a preparation environment for nucleic acid delivery reagents. In plasmid DNA or RNA experiments, this links routine culture conditions with the manipulation step. Using one consistent medium across these activities can help standardize workflows and improve reproducibility.
Applications include routine mammalian cell handling, plasmid DNA transfection, RNA transfection, and optimization of gene-expression experiments. The medium is relevant whenever researchers need cells maintained in a consistent environment while introducing nucleic acids or evaluating expression outcomes. These uses connect day-to-day cell culture with targeted biological manipulation.
The main outcome is a more consistent experimental setting, rather than a guarantee of higher expression. By combining a balanced nutrient and salt environment with reduced serum exposure, Opti-MEM can help limit serum-related variation and support repeatable cell-based measurements. Researchers can use that consistency when interpreting transfection or gene-expression results.