Anesthetize Lobster Ice

Anesthetizing a lobster with ice is a biological handling technique that uses cold temperatures to reduce movement before observation, dissection, or other procedures. Placing the lobster in an ice-cooled environment lowers body temperature, slowing metabolic activity and neuromuscular function; the animal should remain chilled only for the period supported by the experimental protocol. In biology laboratories, this approach can improve handling control and help standardize procedures involving lobster anatomy, physiology, or tissue collection. Because cold exposure may not eliminate sensory responses, researchers should follow species-appropriate welfare guidelines and verify that the method is suitable for the intended application.

Anesthetize Lobster Ice - Related Videos

Research

JoVE Journal - Biology

Gross Dissection of the Stomach of the Lobster, Homarus Americanus

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Cited by 5 •

2009

We describe the gross dissection of the stomach of the American lobster (Homarus americanus).

A Noninvasive Method For In situ Determination of Mating Success in Female American Lobsters (Homarus americanus)

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Cited by 5 •

2014

Fishery-induced changes to exploited crustacean fisheries, such as the American lobster fishery, could potentially influence their reproductive dynamics, leading to a reduction in mating success. This study's goal was to develop a noninvasive method for ascertaining the mating success of female lobsters that may be physiologically or functionally mature.

Identification of Plant Ice-binding Proteins Through Assessment of Ice-recrystallization Inhibition and Isolation Using Ice-affinity Purification

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Cited by 5 •

2017

This paper outlines the identification of ice-binding proteins from freeze-tolerant plants through the assessment of ice-recrystallization inhibition activity and subsequent isolation of native IBPs using ice-affinity purification.

The Use of High-resolution Infrared Thermography (HRIT) for the Study of Ice Nucleation and Ice Propagation in Plants

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Cited by 27 •

2015

Here we present a protocol that allows one to visualize sites of ice formation and avenues of ice propagation in plants utilizing high resolution infrared thermography (HRIT).

Determining the Ice-binding Planes of Antifreeze Proteins by Fluorescence-based Ice Plane Affinity

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Cited by 48 •

2014

Antifreeze proteins (AFPs) bind to specific planes of ice to prevent or slow ice growth. Fluorescence-based ice plane affinity (FIPA) analysis is a modification of the original ice-etching method for determination of AFP-bound ice planes. AFPs are fluorescently labeled, incorporated into macroscopic single ice crystals, and visualized under UV light.

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