Ccd Camera

A CCD camera is an imaging device that converts incoming light into an electronic image, enabling sensitive, quantitative recording in biological research. Its array of light-sensitive pixels accumulates charge when photons generate electrons, then transfers these charges across the chip for sequential readout and conversion into digital intensity values. In microscopy, CCD cameras capture bright-field, phase-contrast, and fluorescence images of cells, tissues, and microorganisms, often supporting measurements of location, shape, and signal intensity. Their high sensitivity and spatial resolution make them useful for documenting dynamic cellular processes and analyzing low-light biological samples.

Ccd Camera - Related Videos

Research

JoVE Journal - Biochemistry

RGBradford: Protein Quantitation with a Smartphone Camera

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Cited by 1 •

2023

This paper provides a protocol for protein quantification using the Bradford assay and a smartphone as an analytical device. Protein levels in samples can be quantified using color data extracted from a picture of a microplate taken with a smartphone.

Simultaneously Capturing Real-time Images in Two Emission Channels Using a Dual Camera Emission Splitting System: Applications to Cell Adhesion

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Cited by 4 •

2013

Dual camera emission splitting systems for two-color fluorescence microscopy generate real-time image sequences with exceptional optical and temporal resolution, a requirement of certain live cell assays including parallel plate flow chamber adhesion assays. When software is employed to merge images from simultaneously acquired emission channels, pseudocolored image sequences are produced.

Research

JoVE Journal - Engineering
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Determining 3D Flow Fields via Multi-camera Light Field Imaging

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Cited by 7 •

2013

A technique for performing quantitative three-dimensional (3D) imaging for a range of fluid flows is presented. Using concepts from the area of Light Field Imaging, we reconstruct 3D volumes from arrays of images. Our 3D results span a broad range including velocity fields and multi-phase bubble size distributions.

Research

JoVE Journal - Medicine
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Semi-Automated Planimetric Quantification of Dental Plaque Using an Intraoral Fluorescence Camera

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Cited by 7 •

2023

This study presents a semi-automated digital image analysis procedure for the planimetric quantification of disclosed dental plaque based on images acquired with an intraoral fluorescence camera. The method allows for the rapid and reliable quantification of dental plaque in the research environment.

Robotized Testing of Camera Positions to Determine Ideal Configuration for Stereo 3D Visualization of Open-Heart Surgery

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2021

The human depth perception of 3D stereo videos depends on the camera separation, point of convergence, distance to, and familiarity of the object. This paper presents a robotized method for rapid and reliable test data collection during live open-heart surgery to determine the ideal camera configuration.

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