Cd31 Cd34 Markers
Cd31 Cd34 Markers - Related Videos
05:32
Purification of CD31+ Endothelial Precursor Cells Using Magnetic-Activated Cell Sorting
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2025
This video demonstrates a technique for purifying CD31+ endothelial progenitor cells (EPCs) from a culture using magnetic-activated cell sorting (MACS). The cell suspension is treated with a fluorophore-conjugated antibody that binds to CD31 on the EPCs. Completing the labeling of CD31+ EPCs involves adding an antibody complex, which binds to the fluorophores, and a polymer-coated magnetic nanoparticle, which binds to the antibody complex. The labeled cells are then purified using a magnetic...
10:28
Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue
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Cited by 8 •
2018
The differentiation of white and beige adipocytes from adipose tissue vascular progenitors bears potential for metabolic improvement in obesity. We describe protocols for a CD34+CD31+ endothelial cell isolation from human fat and for a subsequent in vitro expansion and differentiation into white and beige adipocytes. Several downstream applications are discussed.
08:14
Immunomagnetic Isolation of the Vascular Wall-Resident CD34+ Stem Cells from Mice
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Cited by 1 •
2023
This study has established a stable and efficient method for the isolation, culture, and functional determination of vascular wall-resident CD34+ stem cells (CD34+ VW-SCs). This easy-to-follow and time-effective isolation method can be utilized by other investigators to study the potential mechanisms involved in cardiovascular diseases.
06:57
Leukodepletion Filters-Derived CD34+ Cells As a Cell Source to Study Megakaryocyte Differentiation and Platelet Formation
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Cited by 3 •
2021
This protocol describes in detail all the steps involved in obtaining leukofilter-derived CD34+ hematopoietic progenitors and their in vitro differentiation and maturation into proplatelet-bearing megakaryocytes that are able to release platelets in the culture medium. This procedure is useful for in-depth analysis of cellular and molecular mechanisms controlling megakaryopoiesis.
08:52
Isolation and Enrichment of Liver Progenitor Subsets Identified by a Novel Surface Marker Combination
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Cited by 3 •
2017
Liver injuries are accompanied by progenitor cell expansion that represents a heterogeneous cell population. Novel classification of this cellular compartment allows for the distinguishing of multiple subsets. The method described here illustrates the flow cytometry analysis and high purity isolation of various subsets that can be used for further assays.
FAQs
CD31 is especially informative when researchers examine endothelial organization rather than only identify cells. Its role at endothelial cell junctions links marker detection to both cell adhesion and signaling. Because PECAM-1 supports these functions, examining CD31 expression can help researchers investigate vascular structure during development, tissue repair, or disease-related changes.
CD34 expression provides information about populations associated with hematopoietic progenitors and endothelial cells. Its transmembrane sialomucin character distinguishes its molecular context from CD31's junction-associated adhesion and signaling role. Examining CD34 therefore helps characterize stem and progenitor cell populations while also supporting analyses of endothelial populations in studies of vascular development and tissue repair.
Using both markers can add resolution when a biological sample contains multiple relevant cell populations. Researchers can compare their expression patterns to distinguish populations and relate those populations to endothelial organization or progenitor-associated biology. This paired analysis supports investigations of blood vessel formation, tissue repair, and the relationship between vascular and hematopoietic cell populations.
Flow cytometry uses antibody-based detection to assess CD31 and CD34 expression across cell populations in a biological sample. Researchers can examine the resulting expression patterns to characterize distinct populations and compare their representation within the sample. This approach is particularly useful when the goal is population-level analysis rather than assessment of tissue location or architecture.
Immunohistochemistry and immunofluorescence are useful when the question concerns where marker-positive cells are situated within tissue. By visualizing CD31 or CD34 expression in a tissue context, these antibody-based methods connect cell labeling with organization of vascular structures and surrounding populations. The spatial information complements population characterization in biological samples.
Analysis of these expression patterns supports research on blood vessel formation, vascular development, stem and progenitor cells, tissue repair, and disease-related vascular changes. CD31 can connect observations to endothelial junction organization, while CD34 can help characterize hematopoietic progenitor and endothelial populations. Together, the markers provide complementary context for interpreting cellular and tissue changes in biology.