Cell-based Therapy
Cell-based Therapy - Related Videos
03:52
A Cell-Based Therapy for Autoimmune Arthritis in a Murine Model
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2025
This video showcases a cell-based therapy for autoimmune arthritis in mice. iPSC-derived T regulatory cells expressing ovalbumin-specific receptors are injected with methylated bovine serum albumin (mBSA) emulsified in an adjuvant. Murine antigen-presenting cells (APCs) process and present the mBSA to T cells, prompting T cell activation and migration to the knee joint's synovial membrane. Subsequent injections involve mBSA alone in the left knee and a combination of mBSA and ovalbumin in the...
07:24
Development of an Innovative LED-based Illumination Device for In Vitro Application of Photodynamic Therapy with Rose Bengal
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2025
This manuscript presents a novel, innovative 3D-printed illumination device for studying Rose Bengal-mediated photodynamic therapy in vitro.
06:59
Using the BLT Humanized Mouse as a Stem Cell based Gene Therapy Tumor Model
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Cited by 34 •
2012
The generation and characterization of tumor specific T cells using humanized mice is described here. Human thymic tissue and genetically modified human hematopoietic stem cells are transplanted into immunocompromised mice. This results in the reconstitution of an engineered human immune system allowing for in vivo examination of anti-tumor immune responses.
04:19
Flow Cytometry-Based Quantification of Therapy-Induced Senescent Cancer Cells
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2025
This video illustrates a flow cytometry-based assay for quantifying therapy-induced senescent cancer cells with dual senescence markers. The detection and quantification of senescent cells involve using a β-galactosidase substrate. The substrate is cleaved by active lysosomal β-galactosidase within the senescent cells, producing a fluorescent product that, in conjunction with autofluorescent lipofuscin granules, marks the senescent cancer cells.
FAQs
Therapeutic effects can arise through several biological routes. Some administered cells engraft and differentiate into functional tissue, while others release paracrine signals that support repair without becoming the replacement tissue themselves. Certain cell preparations also regulate immune responses, allowing treatment design to target tissue damage, lost function, or disease-associated immune activity.
Cell type and treatment design determine which biological mechanism is most likely to dominate. A selected population may be intended to replace missing tissue, support repair through signaling, or alter immune activity. Consequently, researchers evaluate the relationship between the cells, their preparation, and the target disease rather than assuming every product acts through engraftment.
Cell sourcing, expansion, delivery, survival, and interaction with host tissues are central variables. The source determines the starting cell population, whereas expansion prepares cells for treatment and delivery places them in the intended biological setting. Subsequent survival and host interactions influence whether the desired repair, functional replacement, or immune regulation can persist.
Hematopoietic stem cell transplantation, tissue regeneration, and cancer immunotherapy represent distinct therapeutic goals. Transplantation addresses replacement of lost cellular functions, tissue regeneration focuses on repairing damaged structures, and cancer immunotherapy uses engineered or immune cells to modify disease processes. Comparing these applications helps researchers match cell behavior and treatment design to the clinical objective.
Development studies examine a connected sequence of considerations: sourcing an appropriate cell population, expanding it, determining how it will be delivered, and evaluating its survival after administration. Researchers also assess interactions with host tissues. Together, these stages help identify whether the cells can produce a useful response while supporting improvements in safety, durability, and effectiveness.
The approach is especially relevant when investigators need to study how living cells repair tissue, replace cellular functions, or regulate disease-related processes. Biology research can examine cell behavior after delivery, including survival and communication with host tissues. These studies support tissue regeneration, cancer immunotherapy, and the development of more personalized regenerative medicine.