Cell Manipulation
Cell Manipulation - Related Videos
13:27
Osteopathic Manipulative Treatment as a Useful Adjunctive Tool for Pneumonia
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Cited by 50 •
2014
Pneumonia is one of the top ten leading causes of death in the U.S. Osteopathic manipulative techniques (OMT) may be utilized as an adjunctive therapy in the treatment of pneumonia to enhance biomechanical and immune function; and ultimately, to reduce hospital stay, duration of antibiotics, incidence of respiratory failure, and mortality. In this video-article, we will review randomized controlled studies on the use of OMT in pneumonia patients, as well as demonstrate specific hands-on...
23:21
Silicon Microchips for Manipulating Cell-cell Interaction
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Cited by 5 •
2007
This article describes an experimental approach for dynamic regulation of cell-cell interactions between adherent cells on a micrometer scale. Manipulation of intercellular communication between hepatocytes and stromal cell is demonstrated. The developed platform enables investigation of cell-cell interactions in a variety of biological processes, including development and pathogenesis.
10:27
Spatio-Temporal Manipulation of Small GTPase Activity at Subcellular Level and on Timescale of Seconds in Living Cells
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Cited by 7 •
2012
A method for spatio-temporal control of small GTPase activity by light is described. This method is based on rapamycin-induced FKBP-FRB heterodimerization and photo-caging systems. Optimization of light-irradiation enables the spatio-temporally controlled activation of small GTPases at the subcellular level.
14:54
Establishment and Genetic Manipulation of Murine Hepatocyte Organoids
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Cited by 3 •
2022
A long-term ex vitro 3D organoid culture system was established from mouse hepatocytes. These organoids can be passaged and genetically manipulated by lentivirus infection of shRNA/ectopic construction, siRNA transfection and CRISPR-Cas9 engineering.
09:49
Generation and Manipulation of Rat Intestinal Organoids
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Cited by 10 •
2023
Here, we present a protocol to generate rat intestinal organoids and use them in several downstream applications. Rats are often a preferred preclinical model, and the robust intestinal organoid system fills the need for an in vitro system to accompany in vivo studies.
FAQs
Viability preserves the cells’ ability to respond and function, while sterility prevents unwanted biological influences from affecting the experiment. Both factors support reliable links between an intervention and the observed cellular response. Maintaining these conditions is especially important when researchers compare altered cells with controls or seek reproducible results for biological and therapeutic studies.
These tools control different aspects of an experiment. Micropipettes help move cells, centrifugation supports separation, and flow sorting enables selection. Chemical treatment can change cellular behavior, whereas gene delivery can alter gene expression or cellular composition. Choosing among them depends on whether the intended outcome concerns location, selection, composition, behavior, or gene activity.
Controlled cellular conditions make it possible to associate a deliberate intervention with a resulting change in cell behavior or properties. Researchers can examine whether altered composition, location, behavior, or gene expression corresponds to a functional outcome. This relationship provides a basis for testing biological mechanisms rather than simply recording that cells changed.
A practical workflow begins by identifying the desired cellular outcome, such as isolation, movement, selection, or alteration. Researchers then choose a compatible tool or treatment, control the cellular conditions, and protect viability and sterility throughout the work. The resulting cells can be examined for changes in properties or function relevant to the research question.
Researchers apply cell manipulation when they need to test how cellular changes influence biological outcomes or when they need cells with selected properties. It supports cell culture, developmental and disease research, drug screening, tissue engineering, and regenerative medicine. The approach is useful both for investigating mechanisms and for developing reproducible biological or therapeutic strategies.
The resulting experiments can reveal how changes in cellular location, composition, behavior, or gene expression relate to function. Depending on the research goal, these observations may clarify developmental or disease processes, support drug screening, or guide tissue engineering and regenerative medicine. Consistent control of conditions also improves the reproducibility of those findings.