Cell Scraper
Cell Scraper - Related Videos
13:42
Clinical Examination Protocol to Detect Atypical and Classical Scrapie in Sheep
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Cited by 23 •
2014
A short clinical examination protocol is presented to aid in the detection of classical and atypical scrapie in sheep and goats.
06:38
Detection of Abnormal Prion Protein by Immunohistochemistry
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2023
Immunolabeling abnormal prion protein using immunohistochemistry protocols requires specific sample and anti-PrP antibody preparation methodologies. The present protocol describes the key steps in epitope demasking to ensure proper PrP immunolabeling and to minimize non-specific background staining. Also, this approach considers biosafety measures when conducting immunohistochemistry studies with the prion-infected tissues.
10:04
Tractable In Vivo Reprogramming of Tumor Cells to Type 1 Conventional Dendritic Cell-like Cells
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2025
This protocol describes the in vivo reprogramming of mouse cancer cells into type 1 dendritic-like cells within the tumor microenvironment through enforced expression of the transcription factors PU.1, IRF8, and BATF3.
11:41
Assessing Transmissible Spongiform Encephalopathy Species Barriers with an In Vitro Prion Protein Conversion Assay
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2015
Measuring the barrier to the interspecies transmission of prion diseases is challenging and typically involves animal challenges or biochemical assays. Here, we present an in vitro prion protein conversion assay with the ability to predict species barriers.
22:06
Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells
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Cited by 7 •
2007
This protocol details the derivation of transplantable hematopoietic stem cells from mouse embryonic stem cells (ESC) and their subsequent injection into lethally irradiated recipient mice. Briefly, ESC are differentiated as embryoid bodies, which are then infected with retroviral HoxB4 and co-cultured with OP9 stromal cells and hematopoietic cytokines.
FAQs
Detachment occurs through controlled mechanical force rather than enzymatic digestion. The flexible blade is pressed against the culture surface and moved across it, disrupting the attachment between the cells and the vessel. This approach can recover cells while avoiding exposure to trypsin or other dissociation enzymes, which is useful when enzyme-free handling is an experimental priority.
Mechanical scraping allows researchers to avoid enzyme exposure during cell recovery. That distinction matters when the experimental design calls for collection without trypsin or another dissociation enzyme. It can also help retain cells together with associated material from the culture surface, supporting analyses in which the recovered material, rather than only isolated cells, is important.
Blade contact and movement determine how mechanical force is applied to the adherent cell layer. Pressing the flexible blade against the culture surface and moving it across the selected area disrupts cell attachment. Controlled handling supports recovery from a defined region, making the process relevant when researchers need localized collection rather than unrestricted sampling of the entire culture surface.
The essential workflow is to position the flexible blade against the culture surface, apply controlled pressure, and move the blade across the area containing adherent cells. The detached material can then be collected for the planned analysis or experiment. Depending on the research goal, recovered cells may be used for counting, microscopy, or molecular analyses.
This technique is especially appropriate when researchers want to recover adherent cells without using trypsin or other dissociation enzymes. It is also suitable when collection must focus on a defined region of a culture dish. Those characteristics make scraping useful for experiments that prioritize enzyme-free handling or spatially targeted recovery from the culture surface.
Recovered material can support several downstream purposes, including cell counting, microscopy, protein extraction, nucleic-acid extraction, and other experiments. Scraping is therefore not limited to simply transferring cells. Because the process can collect cells and associated material from a selected culture region, it can provide samples suited to both observational and molecular analysis.