Core Punching
Core Punching - Related Videos
01:32
Pinching-off of Coated Vesicles
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2023
Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
02:10
The Nucleosome Core Particle
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2020
Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins. The paradox Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their main responsibility is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. While on the other hand, they must allow polymerase enzymes to access DNA...
07:05
A New Workflow for Sampling and Digitizing Increment Cores
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Cited by 11 •
2024
We present a protocol to use 3D-printed mounts to fix increment cores in the field without the necessity of unpacking and gluing them on wooden mounts. The new GSC holder allows placing the cores in a core microtome to cut their surface and directly transfer them to digital image capturing.
02:23
Visualizing the Effect of pH on Solubilization of the Influenza A Viral Core
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2026
Source: Stauffer, S. et al., In Vitro Disassembly of Influenza A Virus Capsids by Gradient Centrifugation. J. Vis. Exp. (2016)This video demonstrates the effect of pH on the solubilization of influenza A viral cores. Gel electrophoresis and protein staining reveal the progressive disassembly of the core structure under acidic conditions.
06:56
Tree Core Analysis with X-ray Computed Tomography
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Cited by 10 •
2023
Here we show how to process tree cores with an X-ray computed tomography toolchain. Except for chemical extraction for some purposes, no further physical lab treatment is needed. The toolchain can be used for biomass estimations, for obtaining MXD/tree-ring width data as well as for obtaining quantitative wood anatomy data.
FAQs
Accurate alignment with the selected tissue region determines whether the extracted core represents the intended morphology or biomarker area. The hollow punch or biopsy needle must be positioned over a defined region before being driven through the specimen. Consistent placement helps produce uniform cores and supports reliable comparison among multiple tissue samples.
Preserving the surrounding material allows the remaining specimen to undergo additional processing, sectioning, or analysis after a selected region has been removed. This is particularly valuable when tissue is limited or contains several areas of biological interest. The approach therefore supports tissue conservation while retaining access to morphology and disease-associated changes outside the sampled core.
Cores from different specimens or regions can be placed into a common recipient block for tissue microarray construction. Examining them under consistent staining and imaging conditions reduces variation caused by separate processing environments. This organization makes it easier to compare tissue morphology, protein expression, biomarkers, and disease-associated changes across the selected samples.
A workflow begins by identifying and marking the tissue region of interest. The hollow punch or biopsy needle is aligned with that area and driven through the specimen to capture a cylindrical core. The core can then be transferred for processing, sectioning, or placement in a recipient block, depending on the planned analysis.
Researchers may use the technique when they need to select defined tissue regions for organized comparative analysis while conserving the larger specimen. In histology and pathology, it is useful for assembling tissue microarrays that contain many specimens or regions. These arrays support parallel examination of morphology, protein expression, biomarkers, and disease-related tissue changes.
Prepared tissue cores can support examination of cellular and structural morphology, protein expression, biomarker distribution, and disease-associated changes. Because multiple cores can be examined under consistent staining and imaging conditions, the resulting data are suited to comparisons among specimens or selected tissue regions. Interpretation depends on the biological features retained within each sampled core.