Embryo Explantation
Embryo Explantation - Related Videos
01:22
Consumer Surplus: Graphical Explanation
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2025
Consumer surplus helps quantify the benefits consumers derive from purchasing goods or services at a price lower than what they are willing to pay. In a market, there are numerous consumers who purchase a product. Different consumers place different values on the same product. For example, consider three shoppers buying a jar of honey. The market price of the jar is $10 per unit. Alice, who values the honey at $20, has a consumer surplus of $10. Ben, willing to pay $18, enjoys a surplus of $8.
01:22
Producer Surplus: Graphical Explanation
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2025
Producer surplus is the difference between the price at which producers are willing to sell their product in the market and the price that they receive. It represents the benefit that producers receive when they sell the product at a higher price than their minimum acceptable price. The supply curve represents the minimum acceptable price for selling each quantity of the good.When all goods are sold at the same market price, the producer surplus is represented as the triangular area between the...
09:08
Facial Transplants in Xenopus laevis Embryos
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Cited by 9 •
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A technique for transplanting "Extreme Anterior Domain" facial tissue between Xenopus laevis embryos has been developed. Tissue can be moved from one gene expression background into another, allowing the study of local requirements for craniofacial development and for signaling interactions between facial regions.
07:32
Protocols for Obtaining Zygotic and Somatic Embryos for Studying the Regulation of Early Embryo Development in the Model Legume Medicago truncatula
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Cited by 2 •
2015
The goal is to illustrate that the model legume Medicago truncatula can be readily utilized to investigate the regulation of early plant embryogenesis to complement the non-legume Arabidopsis model. Pod morphology is linked to zygotic embryogenesis stages and a protocol to collect embryos using tissue culture is also provided.
09:40
Whole Retinal Explants from Chicken Embryos for Electroporation and Chemical Reagent Treatments
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Cited by 6 •
2015
This protocol describes a method to dissect, experimentally manipulate and culture whole retinal explants from chicken embryos. The explant cultures are useful when high success rate, efficacy and reproducibility are needed to test the effects of plasmids for electroporation and/or reagent substances, i.e., enzymatic inhibitors.
FAQs
The nutrient medium provides the support needed for an isolated embryo to remain viable and continue growing outside its original tissues. Depending on the experimental purpose, it may also support regeneration into a complete plant. Observing whether embryos survive, develop, or regenerate helps researchers evaluate the suitability of culture conditions and the biological requirements of embryonic growth.
Separating the embryo from surrounding maternal tissues allows researchers to examine development under controlled laboratory conditions rather than within the original reproductive structure. This isolation makes it possible to investigate how nutritional and hormonal requirements affect embryonic growth. The approach therefore links developmental outcomes to selected culture conditions and reduces the influence of tissues that would otherwise remain associated with the embryo.
Researchers can culture isolated embryos under controlled conditions while examining how nutritional and hormonal support affects survival, growth, or regeneration. These outcomes provide experimental evidence about which requirements are important during development. Because the embryo is separated from its original tissues, changes observed in culture can be related more directly to the conditions being studied.
Assessment may focus on whether the isolated embryo survives, continues growing, or regenerates into a complete plant. These outcomes provide different levels of biological information, from basic viability to the capacity for organized plant development. Comparing such responses under laboratory conditions helps researchers study embryonic development and determine whether culture conditions support recovery or further growth.
A typical workflow begins with aseptically removing the embryo from a seed or another reproductive structure. The isolated material is then placed on a nutrient medium and maintained under controlled laboratory conditions. Researchers monitor survival, growth, and, where relevant, regeneration into a complete plant. This sequence connects careful isolation with subsequent culture-based observation.
Embryo rescue is useful when seeds fail to mature normally but the embryo may still be recoverable. Culturing the embryo separately can provide conditions that support its survival and development, potentially allowing a plant to be obtained despite unsuccessful seed maturation. This makes the technique relevant to recovering valuable plant material that might otherwise be lost.
In plant breeding, the technique can help recover valuable hybrid plants whose seeds may not mature normally. It also supports the study or propagation of plants that are otherwise difficult to propagate, provided the isolated embryo responds successfully to culture. The resulting plants and developmental responses can therefore contribute both to breeding objectives and to biological investigations of plant regeneration.