Flow Cell Assembly
Flow Cell Assembly - Related Videos
11:36
Assembly, Loading, and Alignment of an Analytical Ultracentrifuge Sample Cell
0 Views •
Cited by 21 •
2009
The analytical ultracentrifuge (AUC) sample cell holds sample and reference buffer and during experiments and is exposed to high vacuum and rotor speeds up to 60,000 rpm. This video will demonstrate the rigorous attention to detail necessary for assembly, loading and alignment of this very important component of an AUC experiment.
08:32
Assembly and Characterization of an External Driver for the Generation of Sub-Kilohertz Oscillatory Flow in Microchannels
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2022
The protocol demonstrates a convenient method to produce harmonic oscillatory flow from 10-1000 Hz in microchannels. This is performed by interfacing a computer-controlled speaker diaphragm to the microchannel in a modular manner.
10:43
Flow Cytometry Purification of Mouse Meiotic Cells
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Cited by 57 •
2011
An efficient method to obtain highly purified viable meiotic fractions from mouse testis is described, which combines a refined cell dissociation protocol with fluorescent activated cell sorting (FACS). This method takes advantage of differences in the DNA content and nuclear density of discrete meiotic fractions.
13:05
Micropatterning and Assembly of 3D Microvessels
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Cited by 12 •
2016
This manuscript presents an injection molding method to engineer microvessels that recapitulate physiological properties of endothelium. The microfluidic-based process creates patent 3D vascular networks with tailorable conditions, such as flow, cellular composition, geometry, and biochemical gradients. The fabrication process and examples of potential applications are described.
05:58
Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry
0 Views •
Cited by 16 •
2019
This article describes a FRET-based flow cytometry protocol to quantify protein self-assembly in both S. cerevisiae and HEK293T cells.
FAQs
Accurate alignment keeps the channel geometry continuous across the assembled layers, allowing liquid to move through the intended path. Misalignment can narrow or obstruct the passage and make delivery less consistent. For biological experiments, maintaining a uniform pathway supports repeatable exposure of cells or samples to reagents, nutrients, or wash solutions.
Inlet and outlet ports establish the entry and exit points needed to direct liquids through the defined chamber or microfluidic channel. Their placement and connection must work with the aligned layers and sealed structure. Correctly integrated ports support controlled delivery, washing, and removal of fluids during biological measurements and cell-based experiments.
Bonding or clamping holds the channel layers together and helps maintain a sealed fluid path during operation. An inadequate connection can permit leaks or allow unwanted changes in the channel pathway, whereas a reliable seal supports consistent liquid handling. This stability is important when experiments require repeatable reagent delivery, imaging, or cell culture.
Air bubbles and blockages interrupt the intended liquid pathway and can make delivery through the chamber uneven. These defects may reduce consistent contact between biological samples and flowing reagents, nutrients, or wash solutions. Checking for both during testing helps protect the quality of imaging, cell culture, biochemical assays, and other flow-based measurements.
After the layers, ports, and connections are assembled, the device should be tested under controlled flow. The key checks are whether the structure leaks, whether the channel remains free of blockages, and whether unwanted air bubbles are present. Passing these checks indicates that the assembly can support the planned biological fluid-handling workflow.
An assembled flow cell can support imaging, cell culture, biochemical assays, and other measurements that require liquids to move through a defined region. It can deliver cells, reagents, nutrients, or wash solutions while maintaining access to the biological sample. The appropriate use depends on the experiment’s required flow and observation conditions.
Reliable assembly provides controlled fluid handling and helps maintain stable environmental conditions around the biological sample. It also gives researchers efficient access to the sample during flow-based measurements. By reducing leaks, blockages, and unwanted bubbles, a well-tested device helps different experimental runs receive more consistent liquid delivery and treatment.