Fluidic Isolation
Fluidic Isolation - Related Videos
13:36
Scalable Fluidic Injector Arrays for Viral Targeting of Intact 3-D Brain Circuits
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Cited by 9 •
2010
Controlling and analyzing neural circuits in vivo would be facilitated by a technology for delivery of viruses and other reagents to desired 3-dimensional sets of brain regions. We demonstrate customized fluidic injector array fabrication, and delivery of virally-encoded optical sensitizers, enabling optical manipulation of complex brain circuits.
06:26
Maintaining and Assessing Various Tissue and Cell Types of the Eye Using a Novel Pumpless Fluidics System
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Cited by 2 •
2023
Real-time analysis of live tissue yields important functional and mechanistic data. This paper describes the protocols and critical variables to ensure accurate and reproducible generation of data by a novel and pump-free multi-channel fluidics system that maintains and assesses a wide range of tissue and cell models.
12:32
Combining Fluidic Devices with Microscopy and Flow Cytometry to Study Microbial Transport in Porous Media Across Spatial Scales
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Cited by 2 •
2020
Breakthrough curves (BTCs) are efficient tools to study the transport of bacteria in porous media. Here we introduce tools based on fluidic devices in combination with microscopy and flow cytometric counting to obtain BTCs.
06:12
Modeling the Endothelial Glycocalyx Post-Pneumonectomy in a 3D Fluidic Chip - An Approach to Fabricating a Vascular-based Organ-on-Chip System
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2025
Here we present a protocol for constructing an organ-on-chip (OOC) system designed to mimic pneumonectomy. This system investigates the potential correlation between glycocalyx impairment and complications following pneumonectomy.
10:38
Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis
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Cited by 19 •
2013
Contactless dielectrophoresis (cDEP) achieves sorting and enrichment of particles via their intrinsic dielectric properties. Fluidic electrode channels replace metallic electrodes traditional to DEP, suiting cDEP to non-damaging sterile characterization and sorting of biological particles. We demonstrate how to prepare a cDEP microdevice and conduct cell characterization and sorting experiments.
FAQs
Channel geometry directs where each fluid travels, while pressure-driven flow controls the entry and movement of streams through the system. Together, these features help establish defined paths and limit unintended contact between biological samples and reagents. Their coordination is especially important when several fluids must be delivered in parallel or retained in separate reaction regions.
These components provide different forms of physical control over fluid movement. Hydrophobic boundaries help maintain separation at interfaces, membranes divide adjacent fluid regions, and integrated valves regulate when fluids enter, move, or remain confined. Selecting among these features allows a system to preserve distinct samples or reaction compartments under the conditions required by a biological experiment.
Diffusion can allow molecules to move from one fluid region into another even when bulk flow remains separated. Reducing this exchange helps preserve the intended concentration of reagents and prevents unintended interactions between samples. In compartmentalized assays, limiting diffusion supports more reproducible measurements and makes observed molecular or cellular responses easier to attribute to defined local conditions.
A typical workflow establishes separate fluid paths or compartments, introduces samples and reagents through controlled inlets, and uses geometry, boundaries, membranes, or valves to maintain separation during the experiment. The system then supports the intended reaction, culture condition, or analysis while minimizing cross-contamination. The exact configuration depends on whether the goal is delivery, incubation, or parallel processing.
Researchers can apply this approach when cells need to be analyzed or maintained in defined local environments. Separate compartments help deliver selected reagents, reduce interactions between neighboring samples, and support parallel handling of biological conditions. These capabilities are useful for examining cellular behavior while keeping experimental inputs and surrounding fluid conditions more controlled.
It keeps samples and reaction reagents in designated regions so that multiple assays or biochemical processes can proceed with reduced mixing and lower cross-contamination risk. Precise fluid control also permits small-volume reagent delivery and parallel processing. As a result, researchers can compare reactions or molecular measurements across defined compartments while preserving the intended composition of each local environment.