High-throughput Analysis

High-throughput analysis is a set of methods that rapidly processes and measures large numbers of biological samples, molecules, or cellular features to reveal patterns that individual experiments may miss. It typically combines miniaturized assays, automated liquid handling, robotics, and computational data analysis, allowing many reactions or measurements to occur in parallel under standardized conditions. In biology, high-throughput analysis supports genomic, transcriptomic, proteomic, and drug-screening studies by comparing thousands of genes, compounds, or phenotypes efficiently. These approaches accelerate discovery, improve statistical power, and help researchers identify biological mechanisms, disease-associated signatures, and promising therapeutic candidates.

High-throughput Analysis - Related Videos

Research

JoVE Journal - Biology

Precise, High-throughput Analysis of Bacterial Growth

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Cited by 53 •

2017

Quantitative evaluation of bacterial growth is essential to understanding microbial physiology as a systems-level phenomenon. A protocol for experimental manipulation and an analytical approach are introduced, allowing for precise, high-throughput analysis of bacterial growth, which is a key subject of interest in systems biology.

Research

JoVE Journal - Biochemistry
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High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography

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Cited by 14 •

2023

This protocol details high-throughput crystallization screening, ranging from the 1,536 microassay plate preparation to the end of a 6 week experimental time window. Details are included about the sample setup, the imaging obtained, and how users can perform analyses using an artificial intelligence-enabled graphical user interface to quickly and efficiently identify macromolecular crystallization conditions.

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes

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2015

Here we describe a cell-based reporter gene assay as a valuable tool to screen chemical libraries for compounds modulating post-transcriptional control mechanisms exerted through 3’ UTR.

High-throughput Imaging and Analysis Workflow for Evaluating Skin Cell Phenotypes and Proliferation States in Tissue Samples

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Cited by 1 •

2025

The combination of iterative-bleaching-extends-multiplexity (IBEX) and a commercial nucleotide labeling assay (Click-iT EdU) enables the detection and categorization of dividing cell types in highly dynamic processes in fixed frozen murine tissue sections. Furthermore, a novel open-source image processing pipeline provides high-throughput image acquisition and analysis.

Precise and High-Throughput Analysis of Bacterial Growth Using a Microplate Reader

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2025

Source: Kurokawa, M., et al. Precise, High-throughput Analysis of Bacterial Growth. J. Vis. Exp. (2017)This video demonstrates the high-throughput quantification of bacterial growth using a microplate reader. It outlines the steps involved in culture preparation, optical density measurement, and analysis of growth dynamics.

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