Ice-cap Method
Ice-cap Method - Related Videos
11:15
Ice-Cap: A Method for Growing Arabidopsis and Tomato Plants in 96-well Plates for High-Throughput Genotyping
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Cited by 4 •
2011
The Ice-Cap method allows one to grow plants in 96-well plates and non-destructively harvest root tissue from each seedling. DNA extracted from this root tissue can be used for genotyping reactions. We have found that Ice-Cap works well for Arabidopsis thaliana, tomato, and rice seedlings.
09:43
Identification of Plant Ice-binding Proteins Through Assessment of Ice-recrystallization Inhibition and Isolation Using Ice-affinity Purification
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Cited by 5 •
2017
This paper outlines the identification of ice-binding proteins from freeze-tolerant plants through the assessment of ice-recrystallization inhibition activity and subsequent isolation of native IBPs using ice-affinity purification.
08:46
Determining the Ice-binding Planes of Antifreeze Proteins by Fluorescence-based Ice Plane Affinity
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Cited by 48 •
2014
Antifreeze proteins (AFPs) bind to specific planes of ice to prevent or slow ice growth. Fluorescence-based ice plane affinity (FIPA) analysis is a modification of the original ice-etching method for determination of AFP-bound ice planes. AFPs are fluorescently labeled, incorporated into macroscopic single ice crystals, and visualized under UV light.
09:36
The Use of High-resolution Infrared Thermography (HRIT) for the Study of Ice Nucleation and Ice Propagation in Plants
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Cited by 27 •
2015
Here we present a protocol that allows one to visualize sites of ice formation and avenues of ice propagation in plants utilizing high resolution infrared thermography (HRIT).
08:02
A Computer Vision System for the Assessment of Ice Cream Melting Behavior
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2024
Here, we present a protocol based on a computer vision system (CVS) to determine the melting behavior of multi-phase food systems.
FAQs
Melting ice absorbs heat from the surrounding specimen and environment, helping maintain near-freezing conditions rather than allowing temperature to rise. This reduces molecular motion and slows temperature-related changes while the material is being handled or processed. The cooling effect can therefore help preserve the specimen’s condition long enough to support more consistent laboratory work.
Lower temperatures reduce molecular motion and enzymatic activity, which can slow processes that contribute to specimen degradation. This is particularly relevant when cells or tissues must remain stable during handling, microscopy, or dissection. The benefit is temporary rather than absolute, because the specimen still requires controlled exposure and may be harmed if freezing or ice formation occurs.
Protection depends mainly on exposure time, temperature, and the degree of specimen contact with the ice. Controlled conditions can limit degradation, whereas prolonged exposure or unwanted ice formation may damage cellular structures. Monitoring these variables helps maintain cooling without crossing from near-freezing stabilization into conditions that compromise the biological material.
The method is intended to provide controlled, near-freezing cooling rather than prolonged freezing of the specimen. That distinction matters because cooling can reduce enzymatic activity and temperature-related change, while ice formation within or around biological material can damage cellular structures. Researchers therefore need to manage contact and exposure so stabilization does not become harmful freezing.
A suitable procedure centers on applying a controlled ice layer while the specimen is being handled or processed, then limiting exposure to the time needed for stabilization. Temperature and specimen contact should remain controlled throughout the procedure. These precautions help preserve the material during microscopy, dissection, or related work without encouraging prolonged freezing or structural damage.
The ice-cap method can support short-term preservation when biological specimens must remain stable during handling or processing. Its stated uses include microscopy and dissection, as well as other laboratory procedures in which temperature-related changes could reduce consistency. It is most useful when temporary cooling is needed and the specimen can be monitored for excessive exposure or ice formation.
Careful use can minimize degradation and improve consistency across handling or processing steps by limiting temperature-related changes. The method may help maintain the condition of cells or tissues long enough for microscopy, dissection, or other procedures. Outcomes depend on controlling the cooling conditions, because excessive duration or ice formation can instead compromise cellular structure.