Immunolocalization
Immunolocalization - Related Videos
03:38
Immunolocalization of Arabinogalactan Proteins and Pectins in Plant Tissue
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2025
This video demonstrates an immunostaining technique to localize arabinogalactan proteins (AGPs) and pectins in plant tissues. Fixed and resin-embedded plant tissue sections are stained with AGP- and pectin-specific primary and fluorescent secondary antibodies and a cellulose-binding fluorescent dye to reveal their tissue-specific colocalization in the cell wall.
10:14
Fluorescent Immunolocalization of Arabinogalactan Proteins and Pectins in the Cell Wall of Plant Tissues
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Cited by 2 •
2021
This protocol describes in detail how the plant material for immunolocalization of Arabinogalactan proteins and pectins is fixed, embedded in a hydrophilic acrylic resin, sectioned and mounted on glass slides. We show cell wall related epitopes will be detected with specific antibodies.
13:35
Plunge Freezing: A Tool for the Ultrastructural and Immunolocalization Studies of Suspension Cells in Transmission Electron Microscopy
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Cited by 13 •
2017
This manuscript describes an easy-to-use and low-cost cryofixation method for visualizing suspension cells by transmission electron microscopy.
08:56
Working with Auditory HEI-OC1 Cells
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Cited by 55 •
2016
House Ear Institute-Organ of Corti 1 (HEI-OC1) is one of the few mouse auditory cell lines currently available for research purposes. This protocol describes how to work with HEI-OC1 cells to investigate the cytotoxic effects of pharmacological drugs as well as functional properties of inner ear proteins.
08:42
Immunostaining Phospho-epitopes in Ciliated Organs of Whole Mount Zebrafish Embryos
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Cited by 3 •
2016
Techniques are described to immunostain phospho-epitopes in whole zebrafish embryos and then conduct two-color fluorescent confocal localization in cellular structures as small as primary cilia. The techniques for fixing and imaging can define the location and kinetics of the appearance or activation of specific proteins.
FAQs
Selectivity comes from antibody binding to matching epitopes, the defined regions of a target antigen. Because the signal is tied to that binding, microscopy can map the antigen’s position rather than merely identify its molecular presence. This spatial readout lets investigators relate protein distribution to cellular organization and tissue structure.
Fluorescent, enzymatic, and other labels make antibody binding detectable after the antibody has recognized its target. The label therefore converts a molecular interaction into a visible signal that can be examined by microscopy. Different detection formats support visualization of antigen locations while preserving the method’s focus on spatial distribution.
Fixation helps preserve the spatial context of cells, tissues, or organisms during sample preparation. Maintaining that context is essential because immunolocalization aims to connect a detected antigen with its location in biological structure. Without preserved organization, the resulting signal would provide less useful information about cellular arrangement or tissue-specific distribution.
A basic workflow begins by preparing and fixing the biological sample so its structure is preserved. Antibodies are then used to bind the selected antigen, and the bound antibody is detected through a fluorescent, enzymatic, or other label. Microscopy finally reveals where the signal occurs within the prepared cells, tissue, or organism.
The method can show how a protein or other antigen is distributed across cells, tissues, or organisms. It can also reveal cellular organization and tissue-specific expression, making it possible to connect molecular identity with physical structure. These observations provide spatial information that supports interpretation of cell and tissue function.
Immunolocalization is useful when researchers need to examine how antigen distribution changes across development or in association with disease. Comparing spatial patterns can show where a target is expressed and whether its location differs among biological contexts. Such results help relate molecular changes to tissue organization and broader biological processes.