Lcms Analysis
Lcms Analysis - Related Videos
10:39
Automated Analysis of Intracellular Phenotypes of Salmonella Using ImageJ
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Cited by 3 •
2022
Salmonella invades and replicates inside intestinal epithelial cells both in Salmonella-specific vacuoles and free in the cytosol (hyper-replication). A high-throughput fluorescence microscopy-based protocol is described here to quantify the intracellular phenotypes of Salmonella by two complementary image analyses through ImageJ, reaching single-cell resolution and scoring.
15:04
A Neuronal and Astrocyte Co-Culture Assay for High Content Analysis of Neurotoxicity
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Cited by 46 •
2009
This article describes a novel protocol and reagent set designed for sensitive measurement of neurotoxic effects of compounds and treatments on co-cultures of neurons and astrocytes using high content analysis. Results demonstrate that high content analysis represents an exciting novel technology for neurotoxicity assessment.
06:24
Preparation of Nanoparticles for ToF-SIMS and XPS Analysis
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Cited by 16 •
2020
A number of different procedures for preparing nanoparticles for surface analysis are presented (drop casting, spin coating, deposition from powders, and cryofixation). We discuss the challenges, opportunities, and possible applications of each method, particularly regarding the changes in the surface properties caused by the different preparation methods.
11:24
High-throughput Imaging and Analysis Workflow for Evaluating Skin Cell Phenotypes and Proliferation States in Tissue Samples
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Cited by 1 •
2025
The combination of iterative-bleaching-extends-multiplexity (IBEX) and a commercial nucleotide labeling assay (Click-iT EdU) enables the detection and categorization of dividing cell types in highly dynamic processes in fixed frozen murine tissue sections. Furthermore, a novel open-source image processing pipeline provides high-throughput image acquisition and analysis.
14:11
Quantification of Hydrogen Concentrations in Surface and Interface Layers and Bulk Materials through Depth Profiling with Nuclear Reaction Analysis
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Cited by 21 •
2016
We illustrate the application of 1H(15N,αγ)12C resonant nuclear reaction analysis (NRA) to quantitatively evaluate the density of hydrogen atoms on the surface, in the volume, and at an interfacial layer of solid materials. The near-surface hydrogen depth profiling of a Pd(110) single crystal and of SiO2/Si(100) stacks is described.
FAQs
Separation in the liquid chromatography stage depends on how each compound interacts with the stationary phase and the solvent. Compounds therefore pass through the system at different points, allowing the instrument to resolve components in a complex biological mixture before mass analysis. This separation improves the ability to associate detected signals with particular sample constituents.
Mass spectrometry adds molecular specificity after chromatographic separation. The separated molecules are ionized, and the instrument measures their mass-to-charge ratios, producing information that supports compound detection, identification, measurement, and structural characterization. Using both stages is valuable because retention behavior and mass-related information provide complementary evidence when biological samples contain many compounds.
LCMS analysis can examine metabolites, lipids, peptides, proteins, and drug compounds because its two stages provide different kinds of information. Liquid chromatography resolves the mixture, while mass spectrometry evaluates the resulting ions by mass-to-charge ratio. This combination supports examination of chemically diverse molecules within biological samples rather than focusing on only one compound class.
A biological sample such as blood, tissue, or a cell extract is introduced for chromatographic separation. The separated components then undergo ionization, and their mass-to-charge ratios are measured. The resulting measurements can be used to detect, identify, and measure compounds and to support structural characterization, depending on the biological question.
Biologists use LCMS analysis when they need to examine molecular changes across pathways, identify potential biomarkers, investigate disease mechanisms, or assess responses to treatment. Its application to blood, tissue, and cell extracts connects chemical measurements with biological states. The method can therefore support studies that relate molecular composition to disease processes or therapeutic effects.
LCMS results can provide evidence about which compounds are present, how much of a compound is present, and what structural characteristics may distinguish it. In biological research, these outcomes help organize information about metabolites, lipids, peptides, proteins, or drugs and connect measured molecules with pathways, biomarkers, disease mechanisms, or treatment responses.