Maize Leaf

A maize leaf is the primary photosynthetic organ of the maize plant, capturing light and exchanging gases to support growth, development, and grain production. Its broad blade contains chloroplast-rich mesophyll cells, while veins transport water and minerals into the leaf and distribute sugars to other tissues; stomata regulate carbon dioxide entry and water loss. Studying maize leaves helps researchers examine photosynthesis, plant anatomy, nutrient use, drought responses, and disease development. Because maize is a major crop and a model for grass biology, leaf structure and function provide important insights into crop productivity, environmental adaptation, and agricultural improvement.

Maize Leaf - Related Videos

Research

JoVE Journal - Biology
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Improved Methods for Preparing Transverse Sections and Unrolled Whole Mounts of Maize Leaf Primordia for Fluorescence and Confocal Imaging

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2023

Maize leaf primordia are deeply ensheathed and rolled, making them difficult to study. Here, we present methods for preparing transverse sections and unrolled whole mounts of maize leaf primordia for fluorescence and confocal imaging.

Research

JoVE Journal - Biochemistry

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development

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Cited by 1 •

2017

Many developmentally important genes have cell- or tissue-specific expression patterns. This paper describes LM RNA-seq experiments to identify genes that are differentially expressed at the maize leaf blade-sheath boundary and in lg1-R mutants compared to wild-type. The experimental considerations discussed here apply to transcriptomic analyses of other developmental phenomena.

Research

JoVE Journal - Biology
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Scalable Transfection of Maize Mesophyll Protoplasts

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Cited by 10 •

2023

Here, we present a high-throughput protocol to transiently transfect millions of maize protoplasts for testing large libraries of plasmids in maize mesophyll cells.

Detached Maize Sheaths for Live-Cell Imaging of Infection by Fungal Foliar Maize Pathogens

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Cited by 1 •

2023

This manuscript details an optimized inoculation protocol that uses detached maize leaf sheaths for reproducible cytological, physiological, and molecular studies of maize interactions with fungal plant pathogens. The leaf sheaths facilitate real-time observation of cellular interactions between the living plant and fungus in unfixed tissues.

Lateral Root Inducible System in Arabidopsis and Maize

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Cited by 8 •

2016

The Lateral Root Inducible System (LRIS) allows for synchronous induction of lateral roots and is presented for Arabidopsis thaliana and maize.

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