Multiplexed Biochip
Multiplexed Biochip - Related Videos
06:12
Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
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Cited by 6 •
2023
This paper proposes a new generation of multiparametric analytical platforms with increased throughput for the characterization of extracellular vesicle subsets. The method is based on a combination of multiplexed biosensing methods with metrological and morphomechanical analyses by atomic force microscopy, coupled with Raman spectroscopy, to qualify vesicular targets trapped on a microarray biochip.
11:19
Preparation and Testing of Impedance-based Fluidic Biochips with RTgill-W1 Cells for Rapid Evaluation of Drinking Water Samples for Toxicity
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Cited by 12 •
2016
This manuscript describes how to prepare fluidic biochips with Rainbow trout gill epithelial cells for use in a field portable electric cell-substrate impedance sensor. The protocol for running a rapid drinking water toxicity test with the sensor is also described.
08:03
Multi-analyte Biochip (MAB) Based on All-solid-state Ion-selective Electrodes (ASSISE) for Physiological Research
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Cited by 3 •
2013
All-solid-state ion-selective electrodes (ASSISEs) constructed from a conductive polymer (CP) transducer provide several months of functional lifetime in liquid media. Here, we describe the fabrication and calibration process of ASSISEs in a lab-on-a-chip format. The ASSISE is demonstrated to have maintained a near-Nernstian slope profile after prolonged storage in complex biological media.
04:21
Multiplex Cyclic Fluorescent Immunohistochemistry
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Cited by 1 •
2024
Multiplex cyclic immunohistochemistry allows in situ detection of multiple markers simultaneously using repeated antigen-antibody incubation, image scanning, and image alignment and integration. Here, we present the operating protocol for identifying immune cell substrates with this technology in lung cancer and paired brain metastasis samples.
05:25
Simultaneous Detection of Different Antibody Classes in a Multiplexed Serological Test
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2023
A three-channel dual-reporter fluorescence flow analysis system was used to develop a bead-based multiplex immunoassay that simultaneously evaluates serum samples for IgG and IgM elicited against multiple antigens of different Borrelia species that cause Lyme borreliosis in Europe and North America.
FAQs
Multiplexed biochips distinguish targets by assigning different probes, antibodies, nucleic acids, or cells to separate sensing regions. Each region selectively captures its intended analyte, while optical, electrical, or chemical signals report the resulting detection event. A specialized reader then interprets the combined signals according to their spatial positions, allowing researchers to compare multiple target-specific measurements from one sample.
Integrated microfluidics controls how a biological sample moves across the sensing regions and can reduce reagent use. Controlled delivery helps expose the array to biological material in an organized way while conserving sample-associated reagents. This integration supports miniaturized analysis and is particularly useful when researchers need several measurements from a limited sample.
A multiplexed biochip measures several biological targets within one analytical run instead of focusing on one target at a time. This broader readout can reveal relationships among biological molecules that a single-target measurement may miss. It also increases the information obtained from a small sample and supports higher-throughput analysis when researchers need a coordinated molecular profile.
The platform can generate optical, electrical, or chemical signals from its individual sensing regions. Because each region is associated with a particular probe, antibody, nucleic acid, or cell, the spatial pattern of signals preserves target-specific information. A specialized reader combines and interprets these signals, producing a multi-target readout rather than a single measurement.
A typical workflow begins by delivering a biological sample through the chip, allowing different analytes to interact with designated sensing regions. The resulting signals are then collected and interpreted by a specialized reader. Integrated microfluidics may regulate sample delivery and conserve reagents. This sequence converts simultaneous target capture into a combined analytical readout for biological analysis.
Researchers can apply multiplexed biochips to gene expression analysis, protein profiling, pathogen detection, biomarker studies, and drug screening. The suitable application depends on which biological targets are placed in the sensing array. Across these areas, simultaneous measurements help generate broader molecular information, support comparisons among targets, and reveal relationships that isolated measurements may overlook.