Placenta Dissection
Placenta Dissection - Related Videos
14:42
Isolation and Analysis of Hematopoietic Stem Cells from the Placenta
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Cited by 11 •
2008
We have identified the placenta as a major hematopoietic organ during development. We found that hematopoietic stem cells (HSCs) are both generated and expanded in the placenta in unique microenvironmental niches. Here, we describe experimental techniques required for isolation and visualization of HSCs in the mouse placenta.
12:40
Isolation and Expansion of Mesenchymal Stem/Stromal Cells Derived from Human Placenta Tissue
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Cited by 89 •
2016
Herein we describe methods for the dissection of fetal and maternal tissues from human term placenta, followed by isolation and expansion of mesenchymal stem/stromal cells (MSC) from these tissues.
08:45
Quantitating Iron Transport Across the Mouse Placenta In Vivo Using Nonradioactive Iron Isotopes
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Cited by 3 •
2022
This article demonstrates how to prepare and administer transferrin-bound nonradioactive isotopic iron for studies of iron transport in mouse pregnancy. The approach for quantifying isotopic iron in fetoplacental compartments is also described.
05:31
Disruption of the Mouse Blood-Brain Barrier by Small Extracellular Vesicles from Hypoxic Human Placentas
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Cited by 8 •
2024
A protocol is presented to evaluate whether small EVs (sEVs) isolated from placental explants cultured under hypoxic conditions (modeling one aspect of preeclampsia) disrupt the blood-brain barrier in nonpregnant adult female mice.
07:06
Isolation and Characterization of Mesenchymal Stromal Cells from Human Umbilical Cord and Fetal Placenta
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Cited by 168 •
2017
Here, we present a protocol for the dissection of human umbilical cord (UC) and fetal placenta sample into cord lining (CL), Wharton's jelly (WJ), cord-placenta junction (CPJ), and fetal placenta (FP) for the isolation and characterization of mesenchymal stromal cells (MSCs) using the explant culture technique.
FAQs
Comparing the fetal and maternal surfaces helps reveal how placental organization relates to its two-sided role. Examination of these surfaces, together with the membranes and umbilical cord, provides anatomical evidence about the interface supporting maternal-fetal exchange. In biology, this comparison connects visible structural features with oxygen and nutrient transfer rather than treating the placenta as uniform tissue.
Serial sections expose the internal arrangement of placental tissue and help identify sites of maternal-fetal exchange. A sequence of sections provides a more complete view than examining only an external surface, allowing investigators to relate internal organization to oxygen and nutrient transfer, fetal growth, pregnancy complications, and developmental outcomes.
Observed structural organization can be interpreted in relation to the placenta’s support of fetal development. Dissection allows investigators to connect tissue arrangement and exchange sites with oxygen and nutrient transfer, fetal growth, and developmental outcomes. This relationship makes structural observations useful when considering how pregnancy complications, disease, or injury may affect development.
The examination begins by inspecting the fetal and maternal surfaces, followed by the membranes and umbilical cord. These external and associated structures are assessed before serial sections are taken through the placental tissue. Following this sequence preserves a broad anatomical context and helps relate surface observations to the internal organization revealed later.
In reproductive biology, the method provides direct anatomical evidence of how a temporary organ supports fetal development. In pathology, examining tissue organization, membranes, surfaces, and the cord helps relate placental findings to disease or injury. These observations can then be considered alongside pregnancy complications and developmental outcomes to investigate possible structural associations.
Placenta dissection turns an otherwise temporary organ into a direct subject for studying anatomy, development, and health. Students and researchers can observe the fetal and maternal sides, membranes, cord, and internal sections, then connect those findings with maternal-fetal exchange. This makes abstract processes such as oxygen and nutrient transfer accessible through physical anatomical evidence.