Sonication Disruption
Sonication Disruption - Related Videos
07:24
Sonication Extraction of Lipid Biomarkers from Sediment
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2023
Source: Laboratory of Jeff Salacup - University of Massachusetts Amherst The material comprising the living "organic" share of any ecosystem (leaves, fungi, bark, tissue; Figure 1) differs fundamentally from the material of the non-living "inorganic" share (rocks and their constituent minerals, oxygen, water, metals). Organic material contains carbon linked to a series of other carbon and hydrogen molecules (Figure 2), which distinguishes it from inorganic material. Carbon's wide valency range...
09:03
Disruption of the Blood-Spinal Cord Barrier Using Low-Intensity Focused Ultrasound in a Rat Model
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Cited by 10 •
2023
Disruption of the blood-spinal cord barrier (BSCB) can be successfully achieved with the intravenous administration of microbubbles and the application of low-intensity focused ultrasound (LIFU). This protocol details the opening of the BSCB using LIFU in a rodent model, including equipment setup, microbubble injection, target localization, and BSCB disruption visualization.
03:22
Low Frequency Ultrasound Application: A Method to Determine the Effect of Sonication on Human Leukemia Cells
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2023
This video describes the selective damage of human leukemia cells by using low frequency ultrasound. This application of low frequency ultrasound in a preclinical in vitro condition demonstrates that cell destruction can be attained in order to examine and compare the effects of sonication on leukemia and normal cells.
10:35
Novel Diagnostics in Revision Arthroplasty: Implant Sonication and Multiplex Polymerase Chain Reaction
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Cited by 16 •
2017
Differential diagnostics in painful arthroplasty is crucial for treatment success. Joint aspiration is routinely performed preoperatively. Multiplex polymerase chain reaction of the joint aspirate and the sonication fluid, a feasible tool for quick pathogen detection from these samples is described.
09:05
Generation and Quantitative Analysis of Pulsed Low Frequency Ultrasound to Determine the Sonic Sensitivity of Untreated and Treated Neoplastic Cells
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2015
Selective damage of human leukemia cells can be achieved through a novel approach of applying low frequency ultrasound both with and without chemotherapeutic pretreatment of leukemic and normal hematopoietic cells.
FAQs
The acoustic field creates cavitation bubbles in the sample liquid. Their rapid formation and collapse generates shear forces and fluid movement near cells, stressing and breaking membrane structures. This mechanical action releases intracellular material, so the extent of disruption depends not only on acoustic energy but also on how long and intensely the sample is treated.
Temperature control protects the contents released by lysis. Sonication conditions can generate heat, and excessive warming may damage sensitive biomolecules even when membrane disruption is effective. Monitoring and controlling temperature therefore helps balance two outcomes: sufficient cell opening for recovery of intracellular material and preservation of the proteins, DNA, or RNA being collected.
Power, duration, and sample volume jointly influence how much mechanical energy reaches the biological material. Increasing treatment intensity or time may improve lysis, but the appropriate setting still depends on the amount of sample being processed. Adjusting these variables together helps avoid insufficient disruption while limiting unnecessary exposure that can promote heating or biomolecule damage.
A controlled workflow begins by identifying the biological sample and its volume, then selecting an ultrasonic probe or bath. The operator sets treatment power and duration while managing temperature during exposure. These parameters should be chosen for the intended result, whether releasing intracellular contents, isolating nucleic acids, extracting proteins, or preparing a homogenized sample.
Researchers can apply the method when intracellular material must be recovered from biological samples. Supported uses include protein extraction, DNA isolation, RNA isolation, organelle preparation, and homogenization. The desired product determines which outcome matters most: released biomolecules for downstream work, separated organelles, or a more uniform microbial or tissue sample.
Sonication disruption is relevant across biology because the same physical treatment can serve different sample goals. Microbial and tissue samples may be homogenized, while cellular contents can be released for protein or nucleic-acid isolation. Organelle preparation represents another use, showing that the technique supports both broad sample processing and recovery of particular intracellular materials.