Stereo Microscope

A stereo microscope is an optical instrument that provides a magnified, three-dimensional view of a specimen, making it useful for examining biological structures too large or opaque for conventional compound microscopy. It uses two separate optical paths to deliver slightly different images to the viewer’s eyes, creating depth perception, while reflected or transmitted light illuminates the specimen. In biology, stereo microscopes support dissections, sorting, manipulation, and observation of insects, plants, tissues, and other intact samples. Their relatively low magnification, broad field of view, and substantial working distance allow researchers and students to study surface features and perform precise procedures without sectioning the specimen.

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Research

JoVE Journal - Cancer Research

Comprehensive Spatial Profiling of Species-agnostic Transcriptomes via Stereo-seq

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2025

This article presents a protocol to perform comprehensive spatial transcriptomic profiling of host, viral, fungal, and microbiome RNA from formalin-fixed paraffin-embedded tissue sections using Stereo-seq, which enables high-resolution mapping of diverse transcriptomes while preserving tissue architecture.

Quantification of Strain in a Porcine Model of Skin Expansion Using Multi-View Stereo and Isogeometric Kinematics

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Cited by 9 •

2017

This protocol uses multi-view stereo to generate three-dimensional (3D) models out of uncalibrated sequences of photographs, making it affordable and adjustable to a surgical setting. Strain maps between the 3D models are quantified with spline-based isogeometric kinematics, which facilitate representation of smooth surfaces over coarse meshes sharing the same parameterization.

Development of New Methods for Quantifying Fish Density Using Underwater Stereo-video Tools

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Cited by 9 •

2017

We describe a new method for counting fishes, and estimating relative abundance (MaxN) and fish density using rotating stereo-video camera systems. We also demonstrate how to use distance from camera (Z distance) to estimate species-specific detectability.

Stereo-Electro-Encephalo-Graphy (SEEG) With Robotic Assistance in the Presurgical Evaluation of Medical Refractory Epilepsy: A Technical Note

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Cited by 20 •

2016

Stereo-electroencephalography (SEEG) aids in localization of epileptogenic zones, however, remains relatively underutilized in the United States. The goal of this abstract is to provide a brief introduction to the technique of SEEG and further a detailed technique of using robotic assistance in the placement of SEEG electrodes.

Robotized Testing of Camera Positions to Determine Ideal Configuration for Stereo 3D Visualization of Open-Heart Surgery

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2021

The human depth perception of 3D stereo videos depends on the camera separation, point of convergence, distance to, and familiarity of the object. This paper presents a robotized method for rapid and reliable test data collection during live open-heart surgery to determine the ideal camera configuration.

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