Sutter Puller
Sutter Puller - Related Videos
05:30
Making Patch-pipettes and Sharp Electrodes with a Programmable Puller
0 Views •
Cited by 22 •
2008
This video shows how to use a programmable puller to make patch pipettes and sharp electrodes for electrophysiology. The same procedure can be used to make a variety of glass tools, including injection needles.
12:20
Sputter Growth and Characterization of Metamagnetic B2-ordered FeRh Epilayers
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Cited by 23 •
2013
A method to prepare epitaxial layers of ordered alloys by sputtering is described. The B2-ordered FeRh compound is used as an example, as it displays a metamagnetic transition that depends sensitively on the degree of chemical order and the exact composition of the alloy.
04:22
Fabrication of Bi2Te3 and Sb2Te3 Thermoelectric Thin Films using Radio Frequency Magnetron Sputtering Technique
0 Views •
Cited by 3 •
2024
The manuscript describes a protocol for radio frequency magnetron sputtering of Bi2Te3 and Sb2Te3 thermoelectric thin films on glass substrates, which represents a reliable deposition method that provides a wide range of applications with the potential for further development.
11:47
Characterization of Surface Modifications by White Light Interferometry: Applications in Ion Sputtering, Laser Ablation, and Tribology Experiments
0 Views •
Cited by 13 •
2013
White light microscope interferometry is an optical, noncontact and quick method for measuring the topography of surfaces. It is shown how the method can be applied toward mechanical wear analysis, where wear scars on tribological test samples are analyzed; and in materials science to determine ion beam sputtering or laser ablation volumes and depths.
08:23
Niobium Oxide Films Deposited by Reactive Sputtering: Effect of Oxygen Flow Rate
0 Views •
Cited by 8 •
2019
Here, we present a protocol for niobium oxide films deposition by reactive sputtering with different oxygen flow rates for use as an electron transport layer in perovskite solar cells.
FAQs
Heating brings the glass capillary to a softened state in which it can deform, while the calibrated pulling force separates the softened material and creates tapered ends. The relationship between these two actions determines how narrowly and precisely the glass is shaped. Controlling both is therefore essential when producing micropipettes for experiments that require consistent access to cells or tissues.
Programmable settings allow the pulling process to be controlled so that the resulting micropipette has a selected tip geometry and resistance. These characteristics influence how the pipette performs during biological measurements or manipulation. Reproducible settings help produce comparable pipettes across experiments, reducing variation that could otherwise complicate interpretation of membrane measurements, intracellular recordings, or microinjection results.
Consistent fabrication reduces differences among pipettes used in related experiments. Because tip shape and resistance are controlled during pulling, repeatable production supports more comparable measurements and manipulations from one preparation to the next. This is particularly valuable in studies of membrane properties, cell signaling, and neural function, where variation in the tool can affect experimental consistency.
A glass capillary is positioned in the instrument, heated until the glass softens, and then subjected to a calibrated pulling force. The force divides the softened capillary and produces narrow, tapered tips. Programmable settings are used to control the resulting geometry and resistance, after which the fabricated micropipettes can be selected for the intended biological experiment.
Fabricated micropipettes support several types of biological work, including patch-clamp electrophysiology, intracellular recording, and microinjection. They can also be used in related cell and tissue studies requiring controlled manipulation or measurement. The appropriate pipette characteristics depend on the intended experiment, since programmable control provides different combinations of tip geometry and resistance.
When used in suitable experimental systems, these micropipettes support investigations of membrane properties, cell signaling, and neural function. Patch-clamp electrophysiology and intracellular recording provide approaches for examining cellular electrical behavior, while microinjection enables controlled delivery into cells. Reliable pipette fabrication strengthens these applications by making the physical tool more consistent across measurements and manipulations.