Zebrafish Model
Zebrafish Model - Related Videos
07:58
Nephrotoxin Microinjection in Zebrafish to Model Acute Kidney Injury
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Cited by 8 •
2016
Renal injuries incurred from nephrotoxins, which include drugs ranging from antibiotics to chemotherapeutics, can result in complex disorders whose pathogenesis remains incompletely understood. This protocol demonstrates how zebrafish can be used for disease modeling of these conditions, which can be applied to the identification of renoprotective measures.
08:13
A Scalable Model to Study the Effects of Blunt-Force Injury in Adult Zebrafish
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Cited by 7 •
2021
We modified the Marmarou weight drop model for adult zebrafish to examine a breadth of pathologies following blunt-force traumatic brain injury (TBI) and the mechanisms underlying subsequent neuronal regeneration. This blunt-force TBI model is scalable, induces a mild, moderate, or severe TBI, and recapitulates injury heterogeneity observed in human TBI.
04:53
Zebrafish Larvae as a Model to Evaluate Potential Radiosensitizers or Protectors
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2022
The zebrafish has recently been exploited as a model to validate potential radiation modifiers. The present protocol describes the detailed steps to use zebrafish embryos for radiation-based screening experiments and some observational approaches to evaluate the effect of different treatments and radiation.
01:31
Zebrafish as an Alternative Vertebrate Model Organism in Biomedical Research
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2026
All animal procedures described here must be conducted in accordance with institutional animal ethics guidelines and approved by IACUC. All procedures must follow the principles of the 3Rs—Replacement, Reduction, and Refinement—and must be performed by trained personnel. Zebrafish (Danio rerio) are widely used as an alternative vertebrate model organism in biomedical research. Their genetic similarity to humans, rapid development, and suitability for large-scale studies make them valuable tools...
02:26
Development of a Zebrafish Larval Infection Model Using Microgavage
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2026
Source: Li, J., et al. Development of a Larval Zebrafish Infection Model for Clostridioides difficile. J. Vis. Exp. (2020).This video demonstrates the microgavage procedure, where an anesthetized zebrafish larva is immobilized in agarose and orally injected with a pathogenic bacterium using a tracer-guided needle. The non-invasive delivery mimics natural infection, enabling analysis of bacterial replication, toxin release, and intestinal epithelial damage.
FAQs
Optical transparency permits imaging of developing structures in the living embryo rather than relying only on observations after development. Researchers can follow organ formation and cellular signaling as these events occur. That visibility supports experiments linking dynamic biological processes to physiological or disease-related findings within the same research system.
Accessible genetics makes it possible to manipulate gene function in zebrafish embryos during early development. Researchers can then relate gene changes to organ formation, physiological processes, disease-related biology, or drug responses. This capability connects molecular interventions with observations in a living vertebrate, supporting mechanistic studies rather than purely descriptive comparisons.
Small size and high reproductive output make zebrafish practical for efficient screening and imaging. Researchers can examine many developing organisms while retaining a living vertebrate context, allowing developmental, physiological, or drug-response questions to be investigated at useful scale. These features support both focused experiments and broader screening studies.
A typical investigation can start with embryos developing outside the mother, allowing direct observation of organ formation. Researchers may then manipulate gene function and examine resulting developmental, physiological, or disease-related findings. This workflow combines controlled biological intervention with observation in a living organism, making it useful for connecting biological mechanisms to outcomes.
Zebrafish research can address cell signaling, regeneration, behavior, toxicology, physiology, disease, and drug responses. The appropriate use depends on whether researchers need to examine a developmental process, a whole-organism response, or effects associated with disease or drugs. This breadth makes the model relevant across multiple branches of biology.
Findings from zebrafish can guide studies in other model organisms and inform biomedical research. Their relevance comes from combining vertebrate biology with accessible observations of development, physiology, disease, and drug responses. Researchers can therefore use the system as a source of biological evidence that helps shape follow-up work beyond zebrafish.