Supernatant Decanting
Supernatant Decanting - Related Videos
05:43
A STAGE Tip Procedure for Desalting and Purification of Peptides
0 Views •
2026
Source:Tia Rizakos1, Jennifer Geddes-McAlister11Molecular and Cellular Biology Department, University of Guelph.This video demonstrates peptide purification through the STAGE Tip procedure. A miniature column containing resin beads bonded to long hydrocarbon chains facilitates hydrophobic interaction-based purification. Upon loading a pre-digested peptide sample into the column, the peptide fragments bind to the resin beads, allowing impurities to pass through. An elution buffer that disrupts...
01:49
An Assay To Evaluate the Anticancer Effects of Lactobacillus Cell-Free Supernatant
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2025
This video demonstrates an in vitro assay to study the anticancer effects of Lactobacillus cell-free supernatant (LCFS). Upon exposing colorectal cancer spheroids to varying doses of LCFS, the interaction with the bacterial metabolites in LCFS induces apoptosis in the cancer cells, disrupting the spheroid structure and leading to cell death in a dose-dependent manner.
03:40
Heparin Affinity Chromatography-Based Baculovirus Purification: A Technique to Isolate Baculovirus From Insect Cell Supernatant
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2025
In this video, we demonstrate the technique to isolate baculovirus from an insect cell supernatant using heparin affinity chromatography. The isolation is facilitated by the affinity between the heparin in the column to the baculovirus envelope glycoprotein.
06:07
Evaluating Cell Death Using Cell-Free Supernatant of Probiotics in Three-Dimensional Spheroid Cultures of Colorectal Cancer Cells
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Cited by 15 •
2020
Here methods are presented to understand anti-cancer effects of Lactobacillus cell-free supernatant (LCFS). Colorectal cancer cell lines show cell deaths when treated with LCFS in 3D cultures. The process of generating spheroids can be optimized depending on the scaffold and the analysis methods presented are useful for evaluating the involved signaling pathways.
08:36
Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction
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Cited by 7 •
2018
Real-time quantitative polymerase chain reaction analysis combined with reverse transcription (RT-qPCR) has been widely used to measure the level of RNA virus infections. Here we present a direct RT-qPCR assay, which does not require an RNA purification step, developed for the quantification of several RNA viruses, including dengue virus.
FAQs
Sedimentation and centrifugation determine how firmly the solid phase is concentrated before removal. Sedimentation creates a settled layer through standing, whereas centrifugation concentrates particles into a pellet. A clearer boundary between liquid and solid generally makes controlled transfer easier and reduces the chance of carrying precipitate into the separated liquid.
Container position controls the path of the liquid as it leaves the vessel. Keeping the settled material undisturbed helps preserve the separation achieved during sedimentation or centrifugation, while controlling the flow reduces the chance that solid enters the transferred fraction. These precautions improve recovery and limit cross-contamination in later steps.
The supernatant may be removed by pouring or by aspiration, so the transfer mode can be selected according to how carefully the liquid must be withdrawn from above the settled material. In either case, the operator must control flow and avoid disturbing the precipitate or pellet. This protects the separation during subsequent handling.
First allow particles to settle or use centrifugation to concentrate them at the bottom. Next remove the relatively clear liquid by careful pouring or aspiration, maintaining a controlled flow and avoiding contact with the precipitate or pellet. Finally, manage residual liquid carefully to limit solid loss before filtration, drying, analysis, or reaction.
It is useful when a chemical process produces a liquid phase above a settled solid and the phases must be handled separately. Supported applications include precipitation reactions, solid isolation, washing steps, sample preparation, and purification. The operation can prepare the material for subsequent filtration, drying, analysis, or chemical reaction.
Careful transfer yields a relatively clear liquid while preserving the settled material, supporting reliable downstream handling. Minimizing solid carryover helps reduce cross-contamination, and minimizing solid loss improves recovery. Those outcomes matter whether the next operation is filtration, drying, analysis, or a chemical reaction, because each depends on appropriately separated material.