Peripheral Perfusion

Peripheral perfusion is the delivery of oxygenated blood to tissues outside the heart and central circulation, particularly the skin and extremities, and is an important indicator of circulatory function. It depends on cardiac output, vascular tone, blood volume, and arterial patency; clinicians assess it through skin color and temperature, capillary refill, peripheral pulses, and, when needed, Doppler-based measurements. Evaluating peripheral perfusion helps identify impaired circulation caused by shock, dehydration, vascular obstruction, or reduced cardiac function. In clinical practice, repeated assessment supports early recognition of deterioration, guides treatment, and helps monitor recovery after injury, surgery, or critical illness.

Peripheral Perfusion - Related Videos

Research

JoVE EoE - Rodent Models

Antegrade Perfusion Model: An Ex Vivo Technique to Perfuse Isolated Mouse Heart

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2025

This video describes an antegrade perfusion technique of an isolated mouse heart during which a perfusate is injected through the left ventricle, and eventually, it perfuses the entire myocardium. This method can be used to isolate cardiomyocytes from murine models and generate decellularized hearts for further downstream applications.

Research

JoVE Journal - Bioengineering
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Modified Langendorff Perfusion for Extended Perfusion Times of Rodent Cardiac Grafts

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Cited by 2 •

2024

This article demonstrates the feasibility of achieving longer perfusion times (4 h) of murine cardiac grafts without function loss by employing lower (30-35 mmHg) than physiological (60-80 mmHg) perfusion pressures during Langendorff.

Langendorff Retrograde Heart Perfusion: An Ex Vivo Technique to Perfuse Isolated Mouse Heart

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2025

In this video, we perform Langendorff retrograde perfusion of an isolated mouse heart. During the procedure, the cardiac activity of the heart is maintained by perfusing it retrogradely with a perfusion solution via the coronary arteries using a cannula inserted into the ascending aorta. The model is used to study the cardiac pathophysiology of the heart.

Procurement and Perfusion-Decellularization of Porcine Vascularized Flaps in a Customized Perfusion Bioreactor

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Cited by 4 •

2022

The protocol describes the surgical procurement and subsequent decellularization of vascularized porcine flaps by the perfusion of sodium dodecyl sulfate detergent through the flap vasculature in a customized perfusion bioreactor.

Research

JoVE Journal - Neuroscience
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Whole Animal Perfusion Fixation for Rodents

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Cited by 531 •

2012

Here we describe a low-cost, rapid, controlled and uniform fixation procedure using 4% paraformaldehyde perfused via the vascular system: through the heart of the rat to obtain the best possible preservation of the brain.

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