Cell-laden Bioinks
Cell-laden Bioinks - Related Videos
08:34
Bioprinting Cellularized Constructs Using a Tissue-specific Hydrogel Bioink
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Cited by 59 •
2016
We describe a set of protocols that together provide a tissue-mimicking hydrogel bioink with which functional and viable 3-D tissue constructs can be bioprinted for use in in vitro screening applications.
10:17
Preparation and Characterization of Graphene-Based 3D Biohybrid Hydrogel Bioink for Peripheral Neuroengineering
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Cited by 7 •
2022
In this manuscript, we demonstrate the preparation of a biohybrid hydrogel bioink containing graphene for use in peripheral tissue engineering. Using this 3D biohybrid material, the neural differentiation protocol of stem cells is performed. This can be an important step in bringing similar biomaterials to the clinic.
06:29
Using Multilayered Hydrogel Bioink in Three-Dimensional Bioprinting for Homogeneous Cell Distribution
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Cited by 4 •
2020
Here, we developed a novel multilayered modified strategy for liquid-like bioinks (gelatin methacryloyl with low viscosity) to prevent the sedimentation of encapsulated cells.
11:36
Assembly, Loading, and Alignment of an Analytical Ultracentrifuge Sample Cell
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Cited by 21 •
2009
The analytical ultracentrifuge (AUC) sample cell holds sample and reference buffer and during experiments and is exposed to high vacuum and rotor speeds up to 60,000 rpm. This video will demonstrate the rigorous attention to detail necessary for assembly, loading and alignment of this very important component of an AUC experiment.
02:59
Bead Loading to Introduce Nucleic Acids Into Adherent Cultured Cells: A Technique to Load Fluorescent Protein-Encoding Plasmids Into Adherent Mammalian Cells
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2025
In this video, we describe the procedure to introduce nucleic acids into adherent mammalian cells using bead loading.
FAQs
Viscosity and shear behavior must be balanced so a cell-laden bioink can pass through a bioprinting nozzle, form intended layers, and help protect suspended cells during deposition. These properties connect the formulation’s physical behavior with fabrication quality. Their control therefore influences whether the printed construct can maintain a usable three-dimensional arrangement for later stabilization and biological organization.
Composition determines both the structural support available in the printed construct and the quality of the cell-compatible environment surrounding the cells. A suitable formulation can support cell adhesion, growth, and tissue organization while remaining workable during fabrication. This makes bioink design important not only for producing a shape, but also for supporting biological behavior within that engineered structure.
Crosslinking stabilizes the deposited layers after fabrication, helping the newly formed structure retain its three-dimensional organization. In the workflow, it follows nozzle-based, layer-by-layer deposition rather than replacing that step. This sequence links temporary printability with longer-lasting structural support, allowing the construct to serve as a platform for cell growth, tissue organization, or experimental modeling.
A basic workflow begins by formulating a biomaterial with living cells, followed by depositing it through a bioprinting nozzle in successive layers. The deposited material is then crosslinked to stabilize the construct. Throughout this process, viscosity and shear behavior remain important because they influence deposition and cell protection, while composition supports the intended biological environment.
Researchers may choose these formulations when they need customized three-dimensional biological constructs that combine designed architecture with living cells. The approach is relevant to tissue engineering and regenerative medicine, where cell organization and structural support matter. It also supports disease studies and drug testing by providing engineered models in which biological responses can be investigated within a three-dimensional setting.
These systems can produce customized three-dimensional models that support cell adhesion, growth, and tissue organization. In engineering research, the resulting constructs can be used to study how cells behave within an organized biomaterial environment. Their applications extend from regenerative medicine to disease studies and drug testing, where the construct provides a defined platform for examining biological processes or responses.