Chemical Imaging

Chemical imaging is the spatially resolved detection and mapping of chemical composition, revealing where specific molecules, elements, or materials occur within a sample. It works by collecting position-dependent signals from techniques such as infrared or Raman spectroscopy, fluorescence microscopy, or mass spectrometry, then converting spectral or intensity data into chemical maps. In engineering, these maps help characterize materials, interfaces, coatings, microstructures, and manufacturing processes without relying only on bulk measurements. Chemical imaging can identify contaminants, degradation, defects, and compositional variation, supporting quality control, failure analysis, process optimization, and the design of advanced materials and devices.

Chemical Imaging - Related Videos

Research

JoVE Journal - Chemistry

High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue

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Cited by 2 •

2022

Here, we present a protocol for high-resolution optical detection of chemical information around implanted medical devices with X-ray excited luminescence chemical imaging (XELCI). This novel imaging technique is developed in our lab which enables studying implant-associated infection biochemistry.

Research

JoVE Journal - Biology
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Development of automated imaging and analysis for zebrafish chemical screens.

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Cited by 48 •

2010

We report the development of a system for automated imaging and analysis of zebrafish transgenic embryos in multiwell plates. This demonstrates the ability to measure dose dependent effects of a small molecule, BCI, on Fibroblast Growth Factor reporter gene expression and provide technology for establishing high-throughput zebrafish chemical screens.

Multimodal Nonlinear Hyperspectral Chemical Imaging Using Line-Scanning Vibrational Sum-Frequency Generation Microscopy

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Cited by 2 •

2023

A multimodal, rapid hyperspectral imaging framework was developed to obtain broadband vibrational sum-frequency generation (VSFG) images, along with brightfield, second harmonic generation (SHG) imaging modalities. Due to the infrared frequency being resonant with molecular vibrations, microscopic structural and mesoscopic morphology knowledge is revealed of symmetry-allowed samples.

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

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Cited by 8 •

2022

We present a protocol to acquire chemical images with broadband stimulated Raman scattering (SRS) microscopy. Based on an SRS microscope that operates with differential multichannel-lock-in detection, the protocol describes the sample preparation, adjustment of the SRS apparatus, and chemometrics to disentangle different constituents of chemically heterogeneous samples.

Preparing Adherent Cells for X-ray Fluorescence Imaging by Chemical Fixation

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Cited by 14 •

2015

Here, we present a protocol on how to determine the quantity and distribution of metals in a sample using synchrotron X-ray fluorescence. We focus on adherent cells, and describe the chemical fixation method to prepare this sample. We then describe how to mount and image the sample using synchrotron X-rays.

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