Droplet Formation
Droplet Formation - Related Videos
14:16
Fluorescence detection methods for microfluidic droplet platforms
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Cited by 20 •
2011
Droplet-based microfluidic platforms are promising candidates for high throughput experimentation since they are able to generate picoliter, self-compartmentalized vessels inexpensively at kHz rates. Through integration with fast, sensitive and high resolution fluorescence spectroscopic methods, the large amounts of information generated within these systems can be efficiently extracted, harnessed and utilized.
10:52
Reconstitution of Basic Mitotic Spindles in Spherical Emulsion Droplets
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Cited by 19 •
2016
The assembly and positioning of the mitotic spindle depend on the combined forces generated by microtubule dynamics, motor proteins and cross-linkers. Here we present our recently developed methods in which the geometrical confinement of spherical emulsion droplets is used for the bottom-up reconstitution of basic mitotic spindles.
10:12
A Fluorescence-based Assay for Characterization and Quantification of Lipid Droplet Formation in Human Intestinal Organoids
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Cited by 3 •
2019
This protocol describes an assay for the characterization of lipid droplet (LD) formation in human intestinal organoids upon stimulation with fatty acids. We discuss how this assay is used for quantification of LD formation, and how it can be used for high throughput screening for drugs that affect LD formation.
13:10
Title Cell Encapsulation by Droplets
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Cited by 4 •
2007
07:20
Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy
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2025
Lipophagy is a selective form of autophagy that involves the degradation of lipid droplets. Dysfunctions in this process are associated with cancer development. However, the precise mechanisms are not yet fully understood. This protocol describes quantitative imaging approaches to better understand the interplay between autophagy, lipid metabolism, and cancer progression.
FAQs
Surface tension resists the stretching of a liquid thread or film. An applied flow, pressure, or other force must exceed that resistance sufficiently for the thread to narrow and separate. The balance determines whether pinch-off occurs and helps establish the resulting droplet size and spacing, making surface tension a central control factor in engineered fluid systems.
Droplet size and spacing depend on fluid properties, device geometry, and operating conditions. Changes in these factors alter how far a liquid thread or film stretches before separation and how frequently discrete volumes form. Engineers therefore adjust the fluid, geometry, and imposed conditions together when consistent droplets are required for transport or fabrication.
Applied flow, pressure, or another external force supplies the action needed to deform a liquid against surface tension. If the force does not overcome that interfacial resistance, stretching and separation may not proceed as intended. Controlling the applied force helps engineers regulate the transition from a continuous liquid structure to regularly spaced droplets.
Geometry provides the physical setting in which a liquid thread or film stretches and pinches off, while operating conditions determine how strongly the liquid is driven. Their combined effect influences droplet size and spacing. This relationship allows engineers to design and operate nozzles, inkjet systems, sprays, and microfluidic devices for more consistent fluid control.
A high-level workflow begins by selecting a device suited to the intended process, such as a nozzle, inkjet system, spray, or microfluidic device. Engineers then control the relevant flow, pressure, or other applied force while accounting for fluid properties and geometry. The resulting droplet size and spacing indicate whether the conditions support consistent production.
Engineered droplet production supports printing, coating, chemical processing, and encapsulation. It also contributes to precise transport, mixing, and materials fabrication because discrete liquid volumes can be generated with controlled size and spacing. These uses span industrial and research settings, including systems designed around nozzles, inkjet operation, sprays, and microfluidic devices.