Primary Draw Process

The primary draw process is an engineering manufacturing operation that reduces the cross-sectional area of a metal rod, wire, or tube while increasing its length. A workpiece is pulled through a drawing die, whose smaller opening applies compressive and tensile stresses that produce controlled plastic deformation; die geometry, material properties, and lubrication influence the required force and surface quality. This process establishes the initial dimensions and mechanical condition for subsequent drawing stages. It is widely used to manufacture wire, tubing, and other elongated components with accurate dimensions, improved surface finish, and tailored mechanical properties.

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Education

JoVE Business - Microeconomics

Drawback of Social Welfare Function

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2025

The Rawlsian social welfare function, introduced by philosopher John Rawls, is a framework in welfare economics that assesses societal well-being by focusing on the utility of the least advantaged members. This approach aligns with Rawls's theory of justice, which posits that a just society should prioritize improving the conditions of its most disadvantaged individuals.Consider a society with three individuals:Individual A: Utility level of 8Individual B: Utility level of 4Individual C:...

Research

JoVE Journal - Biology

Tissue Processing and Isolation of Primary Fibroblasts from the Human Vagina

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Cited by 1 •

2024

This protocol demonstrates a reliable and effective technique to isolate primary fibroblasts from either premenopausal or postmenopausal human vaginal tissue. Existing protocols for vaginal fibroblast isolation do not consider the challenges of cell isolation from senescent tissue. Vaginal tissue was obtained from women after pelvic organ prolapse surgery.

Processing of Primary Brain Tumor Tissue for Stem Cell Assays and Flow Sorting

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Cited by 22 •

2012

The identification of brain tumor initiating cells (BTICs), the rare cells within a heterogeneous tumor possessing stem cell properties, provides new insights into human brain tumor pathogenesis. We have refined specific culture conditions to enrich for BTICs, and we routinely use flow cytometry to further enrich these populations. Self-renewal assays and transcript analysis by single cell RT-PCR can subsequently be performed on these isolated cells.

Using Primary Neurosphere Cultures to Study Primary Cilia

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Cited by 3 •

2017

The primary cilium is fundamentally important in neural progenitor cell proliferation, neuronal differentiation, and adult neuronal function. Here, we describe a method to study ciliogenesis and the trafficking of signaling proteins to cilia in neural stem/progenitor cells and differentiated neurons using primary neurosphere cultures.

Primary Neuronal Cultures

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2023

The complexity of the brain often requires neuroscientists to use a simpler system for experimental manipulations and observations. One powerful approach is to generate a primary culture by dissecting nervous system tissue, dissociating it into single cells, and growing those cells in vitro. Primary cultures make neurons and glia easily accessible to the experimental tools required for techniques like genetic manipulation and time-lapse imaging. Furthermore, these cultures represent a highly...

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