Robo-ao System
Robo-ao System - Related Videos
10:35
Bringing the Visible Universe into Focus with Robo-AO
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Cited by 17 •
2013
Light from astronomical objects must travel through the earth's turbulent atmosphere before it can be imaged by ground-based telescopes. To enable direct imaging at maximum theoretical angular resolution, advanced techniques such as those employed by the Robo-AO adaptive-optics system must be used.
07:35
Laser Microdissection-Based Protocol for the LC-MS/MS Analysis of the Proteomic Profile of Neuromelanin Granules
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Cited by 4 •
2021
A robust protocol is presented here for isolating neuromelanin granules from human post-mortem substantia nigra pars compacta tissue via laser microdissection. This revised and optimized protocol massively minimizes the required time for sample collection, reduces the required sample amount, and enhances the identification and quantification of proteins by LC-MS/MS analysis.
08:10
Capture and Release of Viable Circulating Tumor Cells from Blood
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Cited by 2 •
2016
A protocol to utilize a poly(N-iso-propylacrylamide) (PIPAAm) coated microfilter for effective capture and thermoresponsive release of viable circulating tumor cells (CTC) is presented. This method allows capture of CTC from patients' blood and subsequent release of viable CTC for downstream off-chip culture, analyses and characterization.
09:43
Improved Protocol For Laser Microdissection Of Human Pancreatic Islets From Surgical Specimens
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Cited by 25 •
2013
Laser microdissection is a technique that allows the recovery of selected cells from minute amounts of parenchyma. Here we describe a protocol for acquiring human pancreatic islets from surgical specimens to be used for transcriptomic studies. Our protocol improves the intrinsic autofluorescence of human beta cells, thus facilitating their collection.
05:36
Dissection and Lateral Mounting of Zebrafish Embryos: Analysis of Spinal Cord Development
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Cited by 2 •
2014
Developmental processes such as proliferation, patterning, differentiation, and axon guidance can be readily modeled in the zebrafish spinal cord. In this article, we describe a mounting procedure for zebrafish embryos, which optimizes visualization of these events.
FAQs
The laser guide star supplies a reference for measuring wavefront distortions that vary as atmospheric conditions change. Robo-AO uses those measurements to calculate a correction for the deformable mirror, which changes the optical wavefront in real time. Coordinating sensing and correction helps preserve sharper images during observations.
Real-time control matters because the measured wavefront distortion changes while light travels through the atmosphere. A delayed or static correction would not track the current optical error as effectively. By updating the deformable mirror in response to changing measurements, the system maintains its image-sharpening function across an observation and supports consistent spatial resolution.
Robotic control connects target selection, instrument operation, calibration, and data acquisition within one coordinated workflow. This reduces dependence on continuous human intervention and makes observations more efficient and repeatable. For large imaging programs, consistent operating and acquisition practices can be applied across many targets rather than managed as isolated observations.
At a high level, an observation combines target selection, instrument operation, calibration, wavefront measurement with the laser guide star, deformable-mirror correction, and data acquisition. Robotic coordination links these activities with limited human intervention. This integrated sequence allows the platform to produce high-resolution observations through a repeatable observing process.
The system is useful for large imaging surveys, measurements of close stellar companions, and studies of faint or compact objects. These applications depend on consistent high spatial resolution, particularly when important structure or neighboring sources may be difficult to distinguish in blurred images. Automated operation also helps extend the approach across many survey targets.
Repeatability allows observations to follow consistent procedures for target selection, calibration, instrument operation, and data acquisition. In engineering and astronomy, this makes a robotic platform practical for programs requiring many observations rather than a single manually managed measurement. Efficiency and consistency become system-level performance benefits alongside sharper astronomical images.