Shear Cell Sample Environment
Shear Cell Sample Environment - Related Videos
08:19
Shear Assay Protocol for the Determination of Single-Cell Material Properties
0 Views •
Cited by 2 •
2023
This protocol outlines the quantification of the mechanical properties of cancerous and non-cancerous cell lines in vitro. Conserved differences in the mechanics of cancerous and normal cells can act as a biomarker that may have implications in prognosis and diagnosis.
09:20
The Assembly and Application of 'Shear Rings': A Novel Endothelial Model for Orbital, Unidirectional and Periodic Fluid Flow and Shear Stress
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Cited by 10 •
2016
Different levels and patterns of fluid shear are known to modulate endothelial gene expression, phenotype and susceptibility to disease. We discuss the assembly and use of 'shear rings': a model that produces unidirectional, periodic shear stress patterns. Shear rings are simple to assemble, economical and can produce high cell yields.
10:27
Controlled Microfluidic Environment for Dynamic Investigation of Red Blood Cell Aggregation
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Cited by 8 •
2015
The protocol described details an experimental procedure to quantify Red Blood Cell (RBC) aggregates under a controlled and constant shear rate, based on image processing techniques. The goal of this protocol is to relate RBC aggregate sizes to the corresponding shear rate in a controlled microfluidic environment.
07:30
Ensemble Force Spectroscopy by Shear Forces
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Cited by 1 •
2022
Ensemble force spectroscopy (EFS) is a robust technique for mechanical unfolding and real-time sensing of an ensemble set of biomolecular structures in biophysical and biosensing fields.
04:53
A Novel Capsulorhexis Technique Using Shearing Forces with Cystotome
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Cited by 5 •
2010
This video demonstrates a novel capsulorhexis technique in modern cataract surgery. Capsulorhexis is the most difficult and important part of phacoemulsification surgery.
FAQs
A moving surface or tool creates different velocities across the sample, producing a velocity gradient that deforms the material. The resulting shear stress or strain reveals how the sample responds to applied motion rather than simply describing its static properties. This distinction is important when evaluating flow behavior, deformation, or structural changes under controlled loading.
Temperature, atmosphere, cell geometry, and loading conditions can all influence the measured response. Maintaining these variables at defined values helps separate the material’s behavior from changes caused by the test setting. Consistent conditions also make comparisons between fluids, suspensions, polymers, and soft materials more meaningful for engineering interpretation.
The measured response depends on the material and the way it reacts to applied shear. Fluids and suspensions can be examined for flow behavior and viscosity, while polymers and soft materials may show deformation or structural changes. Using the same controlled environment allows these different responses to be interpreted according to the material’s engineering role.
A typical workflow places the material inside the shear cell, establishes the intended geometry, temperature, atmosphere, and loading conditions, and then applies motion through a moving surface or tool. Researchers monitor the response as shear stress or strain develops. The resulting measurements can then be related to flow, deformation, mixing, or structural change.
Depending on the sample and test conditions, the environment can provide information about flow behavior, viscosity, deformation, mixing, and structural changes. These outcomes describe how the material responds while being sheared, giving engineers evidence for comparing formulations or material types and for determining whether laboratory behavior matches a required processing condition.
It is useful when engineers need to connect controlled laboratory measurements with manufacturing, processing, or device-design requirements. Testing fluids, suspensions, polymers, and soft materials under defined conditions can clarify how they behave during applied deformation. The results support interpretation of material properties and help assess suitability for specific engineering operations or designs.