Spatial Profiling

Spatial profiling is the measurement and analysis of how properties, signals, or components vary across physical space, providing context that conventional bulk measurements can obscure. It works by assigning observations to defined locations through imaging, sensor arrays, mapping, or other spatially resolved measurement systems, then linking position with quantitative data to reveal patterns and relationships. In engineering, spatial profiling supports material characterization, device diagnostics, process monitoring, and analysis of complex biological or environmental systems. By identifying local variation, gradients, defects, and interactions, it helps researchers understand system behavior, improve designs, guide quality control, and develop more precise models and interventions.

Spatial Profiling - Related Videos

Research

JoVE Journal - Cancer Research

Comprehensive Spatial Profiling of Species-agnostic Transcriptomes via Stereo-seq

0 Views •

2025

This article presents a protocol to perform comprehensive spatial transcriptomic profiling of host, viral, fungal, and microbiome RNA from formalin-fixed paraffin-embedded tissue sections using Stereo-seq, which enables high-resolution mapping of diverse transcriptomes while preserving tissue architecture.

Research

JoVE Journal - Medicine

Calcified Artery Preparation and Processing with Preserved Morphology and RNA for Digital Spatial Profiling

0 Views •

2026

This protocol includes a step-by-step workflow for calcified vascular specimens: tissue handling, decalcification, RNA validation, calcification level detection, and region-of-interest selection strategies on the Nanostring GeoMx Digital Spatial Profiler (DSP). The goal is to present a comprehensive method for preserving vascular tissue morphology and RNA for reliable spatial transcriptomic analysis.

Digital Spatial Profiling for Characterization of the Microenvironment in Adult-Type Diffusely Infiltrating Glioma

0 Views •

Cited by 1 •

2022

Proteomic dysregulation plays an important role in the spread of diffusely infiltrating gliomas, but several relevant proteins remain unidentified. Digital spatial processing (DSP) offers an efficient, high-throughput approach for characterizing the differential expression of candidate proteins that may contribute to the invasion and migration of infiltrative gliomas.

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

0 Views •

Cited by 2 •

2022

Here, we describe a protocol for fine-tuning regions of interest (ROIs) for Spatial Omics technologies to better characterize the tumor microenvironment and identify specific cell populations. For proteomics assays, automated customized protocols can guide ROI selection, while transcriptomics assays can be fine-tuned utilizing ROIs as small as 50 µm.

Spatial Separation of Molecular Conformers and Clusters

0 Views •

Cited by 3 •

2014

We present a technique that allows the spatial separation of different conformers or clusters present in a molecular beam. An electrostatic deflector is used to separate species by their mass-to-dipole moment ratio, leading to the production of gas-phase ensembles of a single conformer or cluster stoichiometry.

View All Results

FAQs

Related Topics