Dissecting Microscope Setup

Dissecting microscope setup is the process of configuring a stereomicroscope to view and manipulate relatively large, three-dimensional specimens at low magnification, making it useful for environmental observation and analysis. The instrument uses separate optical paths to provide a stereoscopic image, while reflected or transmitted illumination, adjustable magnification, focus, and working distance help reveal surface features and specimen structure. Proper setup supports examination of insects, plant material, soil particles, aquatic organisms, and other environmental samples without extensive preparation. Consistent lighting and stable positioning improve image quality, reduce observation errors, and enable accurate documentation of morphology, damage, contamination, and ecological interactions.

Dissecting Microscope Setup - Related Videos

Research

JoVE Journal - Developmental Biology
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Analysis of Zebrafish Kidney Development with Time-lapse Imaging Using a Dissecting Microscope Equipped for Optical Sectioning

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Cited by 2 •

2016

The method described here allows time-lapse analysis of organ development in zebrafish embryos by using a fluorescence dissecting microscope capable of performing optical sectioning and simple strategies of readjustment to correct focal and planar drift.

Research

JoVE Journal - Biology

Rapid Setup of Tissue Microarray and Tiled Area Imaging on the Multiplexed Ion Beam Imaging Microscope Using the Tile/SED/Array Interface

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Cited by 1 •

2023

Multiplexed ion beam imaging (MIBI) is often used to image tissue microarrays and tiled, contiguous tissue areas, but current software for setting up these experiments is cumbersome. The tile/SED/array interface is an intuitive, interactive graphical tool developed to dramatically simplify and accelerate MIBI run setup.

Kidney Flat Mount Preparation: A Procedure to Dissect and Prepare a Flat Mount for Microscopic Analysis of Adult Zebrafish Kidney

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2023

This video demonstrates the dissection and preparation of a flat mount of the adult zebrafish kidney. The prepared flat mount can be visualized under the microscope for specimen analysis.

A Low Cost Setup for Behavioral Audiometry in Rodents

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Cited by 17 •

2012

A fast and inexpensive method for the behavioral determination of hearing parameters like hearing thresholds, hearing impairments or phantom perceptions (subjective tinnitus) is described. It uses pre-pulse inhibition of the acoustic startle response and can be easily implemented in a personal computer using a programmable AD/DA-converter and a piezo sensor.

Research

JoVE Journal - Neuroscience

Optimizing the Setup and Conditions for Ex Vivo Electroretinogram to Study Retina Function in Small and Large Eyes

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Cited by 5 •

2022

Modification of existing multielectrode array or patch clamp equipment makes the ex vivo electroretinogram more widely accessible. Improved methods to record and maintain ex vivo light responses facilitate the study of photoreceptor and ON-bipolar cell function in the healthy retina, animal models of eye diseases, and human donor retinas.

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