Kernel Screening Assay

A Kernel Screening Assay is a targeted laboratory method for examining individual cereal or plant kernels to identify measurable differences in quality, viability, stress response, or contamination, making it useful for environmental and agricultural assessment. The assay evaluates kernels under defined analytical or biological conditions and compares each sample’s signal with controls to classify affected and unaffected specimens. In environmental research, this screening supports rapid evaluation of exposure effects, contaminant presence, and variation across samples, helping researchers prioritize specimens for confirmatory chemical analysis, ecological studies, or crop-management decisions.

Kernel Screening Assay - Related Videos

Research

JoVE Journal - Immunology and Infection

Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays

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Cited by 36 •

2012

The devastation of cereal crops by seed-infecting fungi has prompted numerous research efforts to better understand plant-pathogen interactions. To study seed-fungal interactions in a laboratory setting, we developed a robust method for the quantification of fungal reproduction, biomass, and mycotoxin contamination using kernel bioassays.

Research

JoVE Journal - Biochemistry
Free Sample

Covalent Fragment Screening Using the Quantitative Irreversible Tethering Assay

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2025

The quantitative irreversible tethering (qIT) assay is a fluorescence-based method of identifying covalent fragments that selectively engage with a target protein. Here, a detailed protocol is outlined to describe the qIT assay, with example results included.

Assay Development for High-Throughput Drug Screening Against Mycobacteria

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2024

This study developed an assay for high-throughput screening against Mycobacterium abscessus with a recently created double reporter strain, using fluorescence and luminescence to assess bacterial viability quickly. This protocol will be relevant for researchers aiming to screen new drugs against this drug-resistant bacteria.

Small Molecule Nematicides Screening Assay: A Medium Throughput Method to Screen Potential Nematicides Against Ditylenchus dipsaci

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2025

In this video, we demonstrate a screening assay to identify the efficacy of small-molecule nematicides against nematodes. Nematicides are considered effective if they cause a decrease in the number of mobile nematodes in the sample after exposure.

High-throughput Functional Screening using a Homemade Dual-glow Luciferase Assay

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Cited by 45 •

2014

We present a rapid and inexpensive screening method for identifying transcriptional regulators using high-throughput robotic transfections and a homemade dual-glow luciferase assay. This protocol rapidly generates direct side-by-side functional data for thousands of genes and is easily modifiable to target any gene of interest.

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