Osmia Ribifloris

Osmia ribifloris is a solitary mason bee native to North America and an important pollinator in natural and managed environments, particularly where blueberries and related plants grow. Females gather pollen and nectar, place the provisions in pre-existing cavities, and divide nests with mud partitions, laying one egg beside each food supply for developing larvae. Because these bees depend on flowering plants, suitable nesting sites, and seasonal environmental conditions, their presence reflects interactions between pollinators and habitat quality. Studying Osmia ribifloris supports pollinator conservation, improves understanding of plant reproduction, and can inform habitat management for resilient ecosystems and agricultural landscapes.

Osmia Ribifloris - Related Videos

Research

JoVE Journal - Neuroscience

Preparing Retinal Organoid Samples for Transmission Electron Microscopy

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Cited by 1 •

2024

This protocol provides an optimized and elaborate preparation procedure for retinal organoid samples for transmission electron microscopy. It is suitable for applications that involve the analysis of synapses in mature retinal organoids.

LPS and ATP-induced Death of PMA-differentiated THP-1 Macrophages and its Validation

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Cited by 1 •

2024

This protocol is based on LPS and ATP-induced death of PMA-differentiated THP-1 macrophages. We use flow cytometry to analyze Annexin V and 7-AAD double staining to detect cell death, using the whole cell and employing scanning electron microscopy to observe the cell membrane morphology.

An Immunolabeling Technique to Visualize the Subcellular Localization of Proteins

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2025

This video demonstrates a technique for the immunolabeling of sigma-1 receptors in a murine heart tissue section using quantum dots. The heart tissue is fixed, osmicated, stained, dehydrated, embedded in resin, and then sectioned. The sections are then treated with antibodies and labeled with quantum dots to demarcate the subcellular localization of Sigma-1 receptors using electron microscopy.

Preparation of Mouse Bladder Tissue for Visualization of Bacterial Infection

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2026

Source: O'Brien, V. P., et. al., Recurrent Escherichia coli Urinary Tract Infection Triggered by Gardnerella vaginalis Bladder Exposure in Mice. J. Vis. Exp. (2020)This video demonstrates the use of scanning electron microscopy to visualize bacterial colonization and host–pathogen interactions in the bladder epithelium of a pre-infected mouse model.

Research

JoVE Journal - Biology
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Visualizing Subcellular Localization of a Protein in the Heart Using Quantum Dots-Mediated Immuno-Labeling Followed by Transmission Electron Microscopy

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Cited by 5 •

2022

The present protocol describes a method of immuno-labeling a protein in the heart tissue sections using quantum dots. This technique provides a useful tool to visualize any protein's subcellular localization and expression at the ultrastructural level.

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