Rapid Sequencing Kit
Rapid Sequencing Kit - Related Videos
12:39
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Here, we present a rapid and cost-effective workflow for characterizing rabies virus (RABV) genomes using nanopore technology. The workflow is intended to support genomics-informed surveillance at a local level, providing information on circulating RABV lineages and their placement within regional phylogenies to guide rabies control measures.
08:43
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07:06
Mouse Sperm Cryopreservation and Recovery using the I·Cryo Kit
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16:02
Demonstration of the Sequence Alignment to Predict Across Species Susceptibility Tool for Rapid Assessment of Protein Conservation
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Cited by 3 •
2023
Here, we present a protocol to utilize the latest version of the US Environmental Protection Agency Sequence Alignment to Predict Across Species Susceptibility (SeqAPASS) tool. This protocol demonstrates the application of the online tool to rapidly analyze protein conservation and provide customizable and easily interpretable predictions of chemical susceptibility across species.
FAQs
The transposase performs two linked tasks: it fragments environmental DNA and attaches sequencing adapters to the resulting pieces. These adapters provide the structures needed for the prepared library to enter a nanopore sequencing device. Combining these operations removes separate preparation stages, allowing the workflow to move from environmental sampling to sequencing more quickly.
The transposase is central because it connects DNA fragmentation with adapter attachment in a single preparation strategy. Fragmentation creates DNA pieces suitable for library preparation, while the attached adapters enable those pieces to enter the sequencing device. This combined action supports a streamlined workflow when environmental analyses require results without extensive processing.
A Rapid Sequencing Kit can prepare a library without the extensive purification or amplification used in some other workflows. Reducing those processing requirements shortens the interval between sample collection and sequencing. That difference is particularly relevant when environmental researchers need timely analysis for microorganism identification, biodiversity assessment, or monitoring changes in environmental DNA.
A common workflow begins with DNA from an environmental sample, followed by transposase-mediated fragmentation and attachment of sequencing adapters. The prepared library then enters the nanopore sequencing device for analysis. Because these preparation actions are combined, the workflow minimizes processing before sequencing and supports a shorter path from sampling to results.
Environmental applications include rapid identification of microorganisms, biodiversity assessment, pathogen surveillance, and monitoring environmental DNA. The approach can be used with samples from water, soil, and other ecosystems. These uses allow sequencing data to contribute to ecological studies while supporting timely examination of biological communities and potential environmental changes.
Its main value is the reduced time between environmental sampling and analytical results. Faster turnaround can help researchers examine microorganisms, biodiversity, pathogens, or environmental DNA while the sampled conditions remain relevant to the investigation. In ecological studies, this timing can strengthen assessments and inform responses to environmental change across different ecosystems.