Stomatal Clustering

Stomatal clustering is the uneven arrangement of stomata, the microscopic pores that regulate gas exchange and water loss on plant surfaces. During leaf development, signaling between neighboring epidermal cells guides stomatal formation and spacing, while environmental conditions such as light, humidity, carbon dioxide, and water availability can influence stomatal distribution and behavior. Clusters may alter the efficiency of carbon dioxide uptake, oxygen release, and transpiration, linking microscopic leaf patterning to plant performance under environmental stress. Studying stomatal clustering helps researchers understand how plants balance photosynthesis with water conservation and how vegetation may respond to changing climates.

Stomatal Clustering - Related Videos

Research

JoVE Journal - Biology
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Relating Stomatal Conductance to Leaf Functional Traits

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Cited by 38 •

2015

Unraveling how physiology and morphology are linked allows a deeper understanding of mechanistic functioning of plant leaves. We present both a procedure to derive parameters of stomatal regulation from stomatal conductance measurements and correlations with traditional functional leaf traits.

Assessing Stomatal Response to Live Bacterial Cells using Whole Leaf Imaging

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Cited by 38 •

2010

We have developed a simple and reproducible protocol to access stomatal response to live bacteria. This method minimizes wounding and manipulation of the leaf as compared to the use of epidermal peels reported previously.

Research

JoVE Journal - Immunology and Infection

Direct Observation and Automated Measurement of Stomatal Responses to Pseudomonas syringae pv. tomato DC3000 in Arabidopsis thaliana

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Cited by 1 •

2024

Here, we present a simple method for direct observation and automated measurement of stomatal responses to bacterial invasion in Arabidopsis thaliana. This method leverages a portable stomatal imaging device, together with an image analysis pipeline designed for leaf images captured by the device.

Research

JoVE Journal - Biology

Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells

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2023

Several commonly used methods are introduced here to study the membrane trafficking events of a plasma membrane receptor kinase. This manuscript describes detailed protocols including the plant material preparation, pharmacological treatment, and confocal imaging setup.

Spatial Separation of Molecular Conformers and Clusters

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Cited by 3 •

2014

We present a technique that allows the spatial separation of different conformers or clusters present in a molecular beam. An electrostatic deflector is used to separate species by their mass-to-dipole moment ratio, leading to the production of gas-phase ensembles of a single conformer or cluster stoichiometry.

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