Vacuum Manipulation
Vacuum Manipulation - Related Videos
13:27
Osteopathic Manipulative Treatment as a Useful Adjunctive Tool for Pneumonia
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Cited by 50 •
2014
Pneumonia is one of the top ten leading causes of death in the U.S. Osteopathic manipulative techniques (OMT) may be utilized as an adjunctive therapy in the treatment of pneumonia to enhance biomechanical and immune function; and ultimately, to reduce hospital stay, duration of antibiotics, incidence of respiratory failure, and mortality. In this video-article, we will review randomized controlled studies on the use of OMT in pneumonia patients, as well as demonstrate specific hands-on...
06:20
Proper Operation of Vacuum Based Equipment
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2023
Source: Robert M. Rioux, Ajay Sathe & Zhifeng Chen, Pennsylvania State University, University Park, PA Vacuum is required for a number of laboratory procedures. This is most routinely achieved in the laboratory by the use of vacuum pumps. In addition to working at low pressures, vacuum pumps can also be used to enable rapid changing of the atmospheres in a reactor or flask by evacuation and backfilling.
03:03
Inoculating a Bacterial Pathogen into Arabidopsis Leaves via Vacuum Infiltration
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2026
Source: Rufián, J. S., et.al. Single-Cell Analysis of the Expression of Pseudomonas syringae Genes within the Plant Tissue. J. Vis. Exp. (2022)This video demonstrates a vacuum infiltration method to introduce a bacterial pathogen into Arabidopsis plant leaves. The approach uses a prepared bacterial suspension and vacuum pulses to enable uniform entry through stomata, resulting in controlled leaf infection.
07:49
Development of an Experimental Setup for the Measurement of the Coefficient of Restitution under Vacuum Conditions
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Cited by 11 •
2016
The coefficient of restitution is a parameter that describes the loss of kinetic energy during collision. Here, a free-fall setup under vacuum conditions is developed to be able to determine the coefficient of restitution parameter for particles in micrometer range with high impact velocities.
23:21
Silicon Microchips for Manipulating Cell-cell Interaction
0 Views •
Cited by 5 •
2007
This article describes an experimental approach for dynamic regulation of cell-cell interactions between adherent cells on a micrometer scale. Manipulation of intercellular communication between hepatocytes and stromal cell is demonstrated. The developed platform enables investigation of cell-cell interactions in a variety of biological processes, including development and pathogenesis.
FAQs
Vacuum pumps create the pressure difference by extracting gas molecules from the chamber, while valves regulate how quickly gas is removed or allowed to move. The sealed chamber preserves the selected condition instead of letting surrounding air immediately replace the extracted gas. Together, these components make pressure adjustment deliberate and repeatable, which is important when experiments depend on controlled gas movement.
Sealing is essential because leaks change the gas content and make the intended pressure difficult to maintain. A chamber that retains its contents allows the pump and valves to establish a defined environment, while controlled adjustment prevents uncontrolled air movement. This stability supports reproducible measurements and reduces the chance that outside gases or contaminants will alter environmentally sensitive samples.
Precise pressure control lets researchers reproduce conditions rather than relying on uncontrolled changes in surrounding air. This matters for experiments involving pressure-dependent processes or atmospheric composition, where differences in gas conditions could affect comparability. In environmental research, controlled settings also help limit contamination, making results easier to interpret and supporting consistent handling of sensitive materials.
A basic workflow begins by securing the material in a sealed chamber and connecting the chamber to a vacuum pump and regulating valves. The pump removes gas, while the valves are adjusted to establish the required pressure condition. Maintaining that selected state during the experiment helps researchers control gas movement and handle samples under defined, repeatable conditions.
It provides a controlled pressure environment for handling materials during preparation, filtration, or drying. The sealed chamber and adjustable valves allow the researcher to establish conditions suited to the task while the pump removes gas. This controlled approach can improve reproducibility, limit contamination, and make environmentally sensitive materials easier to process under defined experimental conditions.
The technique can create defined pressure conditions for gas separation and for studying air or other gases under controlled atmospheric compositions. By limiting uncontrolled exchange with the surroundings, it helps researchers handle gases and compare conditions more consistently. This is relevant when environmental questions involve gas movement, composition, or processes that depend on the surrounding atmosphere.
Environmental researchers and engineers choose Vacuum Manipulation when a task requires controlled pressure, limited contamination, or precise handling of gases and materials. Its applications include sample preparation, filtration, drying, gas separation, and investigating pressure- or composition-dependent processes. These capabilities can improve efficiency and support safer handling of environmentally sensitive materials under experimentally defined conditions.