Fungal Isolation
Fungal Isolation - Related Videos
09:04
Assessing Anti-fungal Activity of Isolated Alveolar Macrophages by Confocal Microscopy
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Cited by 3 •
2014
A method to evaluate the ability of isolated mouse alveolar macrophages to control the growth of phagocytosed Aspergillus spores by confocal microscopy.
08:52
Live-cell Video Microscopy of Fungal Pathogen Phagocytosis
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Cited by 22 •
2013
We describe methods for live-cell video microscopy of Candida albicans phagocytosis by macrophages. These methods enable stage-specific analysis of macrophage migration, recognition, engulfment and phagosome maturation and reveal novel aspects of phagocytosis.
10:33
Isolation of Culturable Yeasts and Molds from Soils to Investigate Fungal Population Structure
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Cited by 7 •
2022
This protocol is an effective, speedy method of culturing yeasts and the mold Aspergillus fumigatus from large sets of soil samples in as little as 7 days. The methods can be easily modified to accommodate a range of incubation media and temperatures as needed for experiments.
10:02
Isolation and Purification of Fungal β-Glucan as an Immunotherapy Strategy for Glioblastoma
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Cited by 1 •
2023
The present protocol describes the purification steps and subsequent studies of four different fungal β-glucans as potential immunomodulatory molecules that enhance the anti-tumoral properties of microglia against glioblastoma cells.
07:31
Microbial and Fungal Diversity - Student Protocol
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2019
Examining Microbial Colony Structures ExpandTo begin, collect your allocated culture plates and then remove the Parafilm and lid from one of them. Place the uncovered plate under a dissecting microscope and then observe the individual communities within each quadrant of the plate. As the colonies are identified, fill out the observed colony properties in the appropriate column in the table. Click Here to download Table 1 Then, repeat the observations for each of the other two bacterial species.
FAQs
Nutrient media and incubation conditions determine which fungi can grow visibly after a sample is processed. Researchers select conditions that support fungal development while limiting bacterial competition, improving the chance of recovering distinct colonies. Because the resulting isolate reflects these laboratory conditions, the chosen media and growth environment can influence which members of an environmental community become available for study.
A pure isolate links later observations to a single cultured organism rather than to a mixed community. Subculturing a distinct colony helps researchers study that organism’s characteristics, including possible roles in decomposition, nutrient cycling, bioremediation, or metabolite production. This separation is therefore essential when interpreting biological activity or comparing fungi recovered from different environmental samples.
The sample source shapes the fungi available for recovery and the way the material must be processed. Soil, water, air, and plant material represent different environmental settings and may contain different fungal communities, along with varying amounts of material that influence culturing. Comparing isolates across these sources can help characterize biodiversity and reveal ecological differences.
A typical workflow begins with collecting an environmental sample, followed by dilution or another form of sample processing. The prepared material is placed on nutrient media and maintained under conditions favorable to fungal growth while bacterial competition is limited. Researchers then distinguish separate colonies and subculture them to obtain pure isolates for subsequent study.
Researchers use fungal isolation when they need cultured organisms from an environment rather than only a general description of the sample. The method supports biodiversity surveys, investigations of decomposition and nutrient cycling, and assessments of contamination or ecological change. It also provides isolates for examining bioremediation, plant–microbe interactions, and environmentally valuable enzymes or metabolites.
The fungi recovered from a sample can contribute to assessments of biodiversity, contamination, and ecological change. Their presence may also support investigations of processes such as decomposition and nutrient cycling. Because isolates can be studied individually, researchers can connect particular cultured organisms with environmental functions or potential applications, including bioremediation and interactions with plants.