Crispr Screening
Crispr Screening - Related Videos
08:32
Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes
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2025
A meticulous and structured approach is given to select resistant and sensitive genes of radiation through the application of a genome-wide CRISPR/Cas9 screen method. This protocol also has the potential to serve as a versatile framework for other research endeavors investigating the mechanisms of resistance to clinically administered chemical drugs.
03:48
Screening for Genomic Modifications: A Method to Identify CRISPR-Generated Mutants in Drosophila
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2023
This video describes a screening method to identify transgenic Drosophila flies, whose genome was modified using CRISPR-Cas9. CRISPR-Cas9 is a powerful genome-editing tool that revolutionized the way scientists can manipulate an organism's genome. In the example protocol, we will see how to identify CRISPR-generated mutants, in which an insertion of a transactivation sequence replaces the first exon of the branchless (bnl) gene, thus creating a bnl gene-specific driver line, bnl-LexA.
01:59
CRISPR
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2019
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
07:37
Performing an In Vitro Genome-Wide CRISPR Knockout Screen in Chimeric Antigen Receptor T Cells
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2025
The article describes a protocol for the application of an in vitro model for exhaustion to complete a genome-wide CRISPR knockout screen in healthy donor chimeric antigen receptor T cells.
02:53
CRISPR and crRNAs
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2020
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea. The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
FAQs
The choice of effector determines whether the screen primarily disrupts genes or changes their regulation. Cas9 can be used to interfere with gene function, whereas dCas9 and other CRISPR effectors support regulatory alterations without necessarily disrupting the underlying DNA sequence. This distinction allows researchers to examine both gene necessity and the consequences of altered gene expression.
Guide RNA libraries provide the collection of distinct genetic perturbations tested across many cells in parallel. Each guide directs a CRISPR effector toward a particular gene or regulatory target, allowing the resulting cellular response to be associated with that perturbation. The breadth of the library determines how extensively the screen can survey genetic contributions to a phenotype.
After cells experience a defined condition, sequencing shows which guide RNAs increase or decrease in representation. Enriched guides identify perturbations associated with improved survival or another selected outcome, while depleted guides indicate perturbations that reduce representation under the same condition. Comparing these patterns links specific genetic changes with condition-dependent cellular traits.
A typical workflow begins by delivering a guide RNA library together with Cas9, dCas9, or another CRISPR effector. Researchers then expose the perturbed cells to a defined selection condition and collect the resulting population for sequencing. They interpret guide enrichment or depletion to identify genes connected with the measured cellular response and prioritize candidates for follow-up studies.
This approach is useful when researchers need to test many genes simultaneously for effects on a disease-related cellular trait, response to a drug, or development of resistance. The resulting perturbation patterns can reveal genes that influence the phenotype and help organize them into functional pathways, providing candidates for mechanistic investigation or therapeutic target evaluation.
In genetics, the combined guide-level results can generate functional maps rather than isolated gene lists. Genes producing related effects under the selected condition may point to shared cellular functions or pathways. These maps help connect genetic perturbations with broader biological processes, identify essential cellular functions, and guide later experiments designed to examine mechanisms in greater detail.