Dry Ice Detachment
Dry Ice Detachment - Related Videos
06:26
Achieving Moderate Pressures in Sealed Vessels Using Dry Ice As a Solid CO2 Source
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Cited by 11 •
2018
Here we present a protocol for performing reactions in simple reaction vessels under low-to-moderate pressures of CO2. The reactions can be performed in a variety of vessels simply by administering the carbon dioxide in the form of dry ice, without the need for costly or elaborate equipment or set-ups.
09:43
Identification of Plant Ice-binding Proteins Through Assessment of Ice-recrystallization Inhibition and Isolation Using Ice-affinity Purification
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Cited by 5 •
2017
This paper outlines the identification of ice-binding proteins from freeze-tolerant plants through the assessment of ice-recrystallization inhibition activity and subsequent isolation of native IBPs using ice-affinity purification.
08:46
Determining the Ice-binding Planes of Antifreeze Proteins by Fluorescence-based Ice Plane Affinity
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Cited by 48 •
2014
Antifreeze proteins (AFPs) bind to specific planes of ice to prevent or slow ice growth. Fluorescence-based ice plane affinity (FIPA) analysis is a modification of the original ice-etching method for determination of AFP-bound ice planes. AFPs are fluorescently labeled, incorporated into macroscopic single ice crystals, and visualized under UV light.
08:02
A Computer Vision System for the Assessment of Ice Cream Melting Behavior
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2024
Here, we present a protocol based on a computer vision system (CVS) to determine the melting behavior of multi-phase food systems.
09:36
The Use of High-resolution Infrared Thermography (HRIT) for the Study of Ice Nucleation and Ice Propagation in Plants
0 Views •
Cited by 27 •
2015
Here we present a protocol that allows one to visualize sites of ice formation and avenues of ice propagation in plants utilizing high resolution infrared thermography (HRIT).
FAQs
Rapid cooling creates thermal shock that freezes adherent cells or biological material in place. This sudden temperature change weakens interactions between the sample and culture surface, allowing the material to be lifted without enzymatic dissociation. The mechanism is therefore physical rather than proteolytic, which matters when researchers want to avoid enzyme exposure during harvesting.
Enzymatic dissociation exposes harvested material to proteolytic enzymes, whereas this technique removes material through rapid cooling and lifting. Avoiding that treatment can help preserve the sample’s composition, an important consideration when recovered cells or biological material will undergo molecular analysis. In genetics, preservation supports subsequent DNA or RNA isolation and related investigations.
The main distinction is the source of detachment: enzyme-based methods rely on proteolytic activity, while Dry Ice Detachment relies on rapid cooling followed by lifting from the culture surface. This difference gives researchers a nonenzymatic option when minimizing treatment-related changes is important. It also makes the approach relevant to workflows that prioritize rapid, gentle sample harvesting.
The workflow begins with adherent cells or biological material in a culture vessel. Solid carbon dioxide is then used to rapidly cool the vessel, producing the freezing and thermal-shock effects that weaken surface attachment. After cooling, the material is lifted from the surface for collection and subsequent processing, such as molecular analysis.
Researchers may select Dry Ice Detachment when they need to recover cellular material for downstream DNA or RNA isolation and want to avoid enzymatic dissociation. Its value is greatest when rapid harvesting and preservation of sample composition are important. The method can therefore fit genetics workflows focused on obtaining material for molecular analyses rather than relying on proteolytic treatment.
Material collected after detachment can support DNA isolation, RNA isolation, and other molecular analyses identified in the workflow. Because the method avoids proteolytic enzymes and can help preserve sample composition, it provides a practical harvesting option for genetics research. The resulting sample is intended for downstream molecular processing, not merely for removing cells from the vessel.